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Anti-CCND1 Antibody EP272Y
Also for CCND1 (NM_053056)
|A synthetic peptide corresponding to residues near the N-terminus of human Cyclin D1 was used as immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; ICC/IF: 1:50 - 1:100; IP: 1:10 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt.
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Western blot - Cyclin D1 antibody [ep272y]; Anti-Cyclin D1 antibody [EP272Y] at 1/2000 dilution + MCF-7 cell lysate at 10 ug.Predicted band size : 33 kDa.Observed band size : 36 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D1 antibody [EP272Y]; Immunohistochemical analysis of paraffin-embedded human kidney carcinoma using anti-Cyclin D1 RabMAb.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D1 antibody [EP272Y]; Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using anti-Cyclin D1 RabMAb.
Immunocytochemistry/ Immunofluorescence - Anti-Cyclin D1 antibody [EP272Y]; TA300599 staining cyclin D1 in MCF7 cells treated with KN-93 (water soluble) , by ICC/IF. Decrease in cyclin D1 expression correlates with increased concentration of KN-93 (water soluble), as described in literature.The cells were incubated at 37Â°C for 24 hours in media containing different concentrations of (KN-93 (water soluble)) in DMSO, fixed with 100% methanol for 5 minutes at -20Â°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with TA300599 (1/100 dilution) was performed overnight at 4Â°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Immunocytochemistry/ Immunofluorescence - Cyclin D1 antibody [ep272y]; Immunofluorescence staining of Cyclin D1 on MCF7 cells, using TA300599 at a dilution of 1/50.