Home Antibody All anti-CCND1 antibodies
Anti-CCND1 Antibody EP272Y
Also for CCND1 (NM_053056)
|A synthetic peptide corresponding to residues near the N-terminus of human Cyclin D1 was used as immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; ICC/IF: 1:50 - 1:100; IP: 1:10 - 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt.
* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
Western blot - Cyclin D1 antibody [ep272y]; Anti-Cyclin D1 antibody [EP272Y] at 1/2000 dilution + MCF-7 cell lysate at 10 ug.Predicted band size : 33 kDa.Observed band size : 36 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D1 antibody [EP272Y]; Immunohistochemical analysis of paraffin-embedded human kidney carcinoma using anti-Cyclin D1 RabMAb.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin D1 antibody [EP272Y]; Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using anti-Cyclin D1 RabMAb.
ICC/IF - Anti-Cyclin D1 antibody; TA300599 staining cyclin D1 in MCF7 cells treated with KN-93 (water soluble) , by ICC/IF. The cells were incubated at 37°C for 24 hours in media containing different concentrations of (KN-93 (water soluble)) in DMSO. Staining of the treated cells with TA300599 (1/100 dilution) was performed overnight at 4°C. A DyLight 488 goat anti-rabbit polyclonal antibody at 1:250 was used as the secondary antibody. Nuclei were counterstained with DAPI.
Immunocytochemistry/ Immunofluorescence - Cyclin D1 antibody [ep272y]; Immunofluorescence staining of Cyclin D1 on MCF7 cells, using TA300599 at a dilution of 1/50.