Home Antibody All anti-MAP2K4 antibodies
Anti-MAP2K4 Antibody EP615Y
Also for MAP2K4 (NM_003010)
|A synthetic peptide corresponding to residues in the C-term of human MKK4 was used as immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||WB: 1:1000; FC: 1:1000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for ICC or IP.
* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
Western blot - SEK1 / MKK4 antibody [EP615Y]; Anti-MEK4 antibody [EP615Y] at 1/1000 dilution + HeLa cell lysate.Predicted band size : 44 kDa.Observed band size : 44 kDa.
Immunohistochemistry - MEK4 antibody [EP615Y]; IHC-P of lung adenocarcinoma using anti-MKK4 (C term) TA300580 at 1/100.
Flow Cytometry - Anti-MEK4 antibody [EP615Y]; Overlay histogram showing K562 cells stained with TA300580 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.