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Anti-MAP2K4 Antibody EP559Y
Also for MAP2K4 (NM_003010)
|A synthetic peptide corresponding to residues near the N-term of human MKK4 was used as immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:500-2,000; IHC: 1:50-100; ICC: 1:250-500; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Does not react with Mouse, Rat
|Homo sapiens mitogen-activated protein kinase kinase 4 (MAP2K4), transcript variant 1|
|JNKK; JNKK1; MAPKK4; MEK4; MKK4; PRKMK4; SAPKK-1; SAPKK1; SEK1; SERK1; SKK1|
|MKK4, also known as JNKK1 and SEK1, is a dual specificity kinase that belongs to the Ser/Thr protein kinase family. MKK4 is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. MKK4 phosphorylate and activate p38 MAP kinase and JNK but not ERK 1/2 (1-2). JNK is activated by dual phosphorylation at Thr and Tyr by MKK4 and MKK7; although MKK4 will preferentially phosphorylate Tyr (3). MKK4 is also known to be a tumor suppressor, as mutations inactivating the MKK4 gene have been found in 5% of a wide variety of tumor types (4). |
MAPK signaling pathway
Toll-like receptor signaling pathway
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Western blot - SEK1 / MKK4 antibody [EP559Y]; Anti-MEK4 antibody [EP559Y] at 1/2000 dilution + Hela cell lysate.Predicted band size : 44 kDa.Observed band size : 44 kDa.Additional bands at : 62 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Paraffin-embedded sections) - SEK1 / MKK4 antibody [EP559Y]; ab33904, at a 1/50 dilution, staining human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue.
Immunofluorescence - SEK1 / MKK4 antibody [EP559Y]; ab33904, staining Hela cells by Immunofluorescent