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Anti-ITK Antibody Y401
|A synthetic peptide corresponding to residues in the N-term of human Emtk/Itk/Tsk was used as immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:1000 - 1:5000; IHC-P: Use at an assay dependent concentration; FC: 1:100
|Does not react with Mouse, Rat. Is unsuitable for IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens IL2-inducible T-cell kinase (ITK)|
|EMT; LPFS1; LYK; PSCTK2|
|Emtk/Itk/Tsk is a member of the Tec family of Protein Tyrosine Kinase (PTK) expressed in T, NK, and mast cells (1). Structurally, Emtk/Itk/Tsk possesses a tyrosine kinase (TK), a pleckstrin homology (PH) and a TEC homology domain. It also contains both SRC homology 2 (SH2) and a SRC homology 3 (SH3) domains (2). It lacks as well the amino-terminal myristylation signal and the negative autoregulatory phosphorylation site present in all members of the Src family of PTKs (3). Additionally, the Src tyrosine kinase LCK is required for Emtk/Itk/Tsk activation and recruitment by CD28 (4). |
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Western blot - ITK antibody [Y401]; Anti-ITK antibody [Y401] at 1/5000 dilution + Jurkat cell lysate.Predicted band size : 72 kDa.Observed band size : 68 kDa .
Immunohistochemistry (Paraffin-embedded sections) - ITK antibody [Y401]; Ab32039 at a 1/250 dilution, staining ITK in paraffin embedded human lymph node tissue by Immunohistochemistry.
Flow Cytometry-Anti-ITK antibody [Y401](ab32039); Overlay histogram showing Jurkat cells stained with ab32039 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (1µg/1x10^6 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.