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Anti-ITK Antibody Y402
|A synthetic peptide corresponding to residues near the C-term of human Emtk/Itk/Tsk was used as immunogen.|
|Human (Does not react with: Mouse, Rat)
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000; FC: 1:50 - 1:1000; IP: 1:50
|Does not react with Mouse, Rat. Is unsuitable for ICC/IF or IHC.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for ICC/IF or IHC.
|Homo sapiens IL2-inducible T-cell kinase (ITK)|
|EMT; LPFS1; LYK; PSCTK2|
Entrez Gene 3702 Human
|Emtk/Itk/Tsk is a member of the Tec family of Protein Tyrosine Kinase (PTK) expressed in T, NK, and mast cells (1). Structurally, Emtk/Itk/Tsk possesses a tyrosine kinase (TK), a pleckstrin homology (PH) and a TEC homology domain. It also contains both SRC homology 2 (SH2) and a SRC homology 3 (SH3) domains (2). It lacks as well the amino-terminal myristylation signal and the negative autoregulatory phosphorylation site present in all members of the Src family of PTKs (3). Additionally, the Src tyrosine kinase LCK is required for Emtk/Itk/Tsk activation and recruitment by CD28 (4). |
|Protein KinaseDruggable Genome Chemokine signaling pathwayT cell receptor signaling pathwayLeukocyte transendothelial migration|
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Western blot - ITK antibody [Y402]; Anti-ITK antibody [Y402] at 1/1000 dilution + Jurkat cell lysate.Predicted band size : 72 kDa.Observed band size : 72 kDa.
Flow Cytometry - Anti-ITK antibody [Y402]; Overlay histogram showing Jurkat cells stained with TA300522 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.