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Anti-PTPN11 Antibody Y478
Also for PTPN11 (NM_002834)
|A synthetic peptide corresponding to residues near the C-term of human SHP-2 was used as immunogen. This antibody is predicted to detect splice isoform 2 based on sequence analysis.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IHC-P: Use at an assay dependent dilution; IP: 1:50
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens protein tyrosine phosphatase, non-receptor type 11 (PTPN11), transcript variant 1|
|BPTP3; CFC; NS1; PTP-1D; PTP2C; SH-PTP2; SH-PTP3; SHP2|
|SHP-2 (also called PTP2C, SH-PTP2, PTP1D, SH-PTP3, Syp and PTPN11) is a protein tyrosine phosphatase (PTP) expressed in most cell types (unlike SHP-1), (1). SHP-2 is involved in t the signal transduction stimulated by EGF, PDGF and Insulin. After cell stimulation, SHP-2 (as well as SHP-1), translocates from cytosol to the plasma membrane and binds to tyrosine-phosphorylated receptors through their SH2 domains, becoming activated in the process (2). When phosphorylated, SHP-2 has been shown to bind Grb2, EGFR, PDGFR, IRS-1 via its SH2 domain (3). Overexpression of SHP-2 leads to Juvenile Myelo-monocytic Leukemia and Noonan syndrome (4-5).|
EGFR1 Signaling Pathway
Jak-STAT signaling pathway
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Western blot - SHP2 antibody [Y478]; Anti-SHP2 antibody [Y478] at 1/5000 dilution + Jurkat cell lysate.Predicted band size : 68 kDa.Observed band size : 70 kDa .
Immunohistochemistry (Paraffin-embedded sections) - SHP2 antibody [Y478]; Immunohistochemical analysis of SHP2 expression in paraffin embedded human breast carcinoma, using 1/50 ab32083.