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Anti-HDAC3 Antibody Y415
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Also for HDAC3 (NM_003883)
|A synthetic peptide corresponding to residues in the C-term of human Histone deacetylase 3 was used as immunogen. This antibody may detect splice isoform 2 (RPD3-2A) according to sequence analysis.|
|Human, Mouse, Rat
|WB, IHC, IF
||WB: 1:5,000-10,000; IHC: 1:250-500; ICC: 1:250-500; IP: 1:50
|50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Homo sapiens histone deacetylase 3 (HDAC3)|
|HD3; RPD3; RPD3-2|
|Histone deacetylase 3 belongs to the histone deacetylase family type 1 which actively deacetylate Histones. Currently 3 known isoforms exists (HDAC3, HDAC3A and HDAC3C) (1). HDAC3 represses transcription and binds transcription factor YY1 to form a large complex. N-Cor binds HDAC3 in a different manner than other HDACs. In addition to N-Cor, HDAC3 will form a complex with corepressor SMRT (2). HDAC3 is also known to interact with DAXX, HDAC10 and DACH1. |
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Western blot analysis on K562 cell lysate using anti-Histone Deacetylase 3 (C-term), 1:10,000
Immunohistochemical analysis of paraffin-embedded human ovary carcinoma using anti-Histone deacetylase 3 (C-term).
Immunofluorescent staining of HeLa cells using anti-Histone deacetylase 3 (C-term).