Home Antibody All anti-BAD antibodies
Anti-BAD Antibody Y208
|A synthetic peptide corresponding to residues in the N-term of human Bad was used as immunogen. The antibody does not cross-react with other Bcl-2 members.|
|Mouse, Rat, Dog, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||WB: 1:2000 - 1:5000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:250 - 1:500; FC: 1:20 - 1:50; IP: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified
* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
Western blot - Bad antibody [Y208]; Anti-Bad [Y208] antibody at 1/5000 dilution + Hela cell lysate.Predicted band size : 18 kDa.Observed band size : 23 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Bad antibody [Y208]; Immunohistochemical staining of paraffin-embedded human lymphoma using TA300475 at 1/100 dilution.
ICC/IF image of TA300475 stained MCF7 cells. The cells were incubated with the antibody overnight at 4.. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at 1:1000 for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
Flow Cytometry-Bad antibody(TA300475); Overlay histogram showing MCF-7 cells stained with TA300475 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. This antibody gave a decreased signal in MCF-7 cells under the same conditions.