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Anti-CHEK2 Antibody Y171
Also for CHEK2 (NM_007194)
|A synthetic phospho-peptide corresponding to residues surrounding Threonine 68 of human Chk2 was used as immunogen. The antibody will detect Chk2 phosphorylation on Threonine 68. This antibody may also detect all splice isoforms (2-12) based on sequence h|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1: 1,000-5,000; IP: 1:50
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Whole Cell Lysate (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens checkpoint kinase 2 (CHEK2), transcript variant 1|
|CDS1; CHK2; hCds1; HuCds1; LFS2; PP1425; RAD53|
|Chk2 is a checkpoint kinase that plays a role in regulating cell cycle progression. In response to DNA damage and DNA replicational stress, Chk1 and Chk2 phosphorylate Cdc25C to prevent entry into mitosis (1,2). Consisting of ionizing radiation (IR), UV irradiation or hydroxyurea treatment, DNA damage induces ATM/ATR to phosphorylate Thr68 and other sites in the amino terminal domain (3). Phosphorylation at Thr68 is a prerequisite for subsequent activation steps.|
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Western blot - Chk2 (phospho T68) antibody [Y171]; All lanes : Anti-Chk2 (phospho T68) antibody [Y171] at 1/1000 dilution.Lane 1 : 293 cell lysate.Lane 2 : 293 cell lysate treated with 2uM Doxorubicin.Lane 3 : 293 cell lysate treated with 10uM Doxorubicin.Predicted band size : 61 kDa.Observed band size : 62 kDa .
Dot Blot - Chk2 (phospho T68) antibody [Y171]; Dot Blot analysis on immunogen phospho-peptide (A) and non-phospho peptide (B) using anti-Phospho-Chkr2 (pT68) RabMAb dilution 1:500