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Anti-JUN Antibody Y172
|A synthetic phospho-peptide corresponding to residues surrounding Ser63 of human c-Jun was used as immunogen. The antibody only detects c-Jun phosphorylated on Serine 63. Predicted to cross-react with bovine, based on sequence homology.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100; ICC: 1:100 - 1:200
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Is unsuitable for Flow Cyt or IP.
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Western blot - c-Jun (phospho S63) antibody [Y172]; All lanes : Anti-c-Jun (phospho S63) antibody [Y172] at 1/10000 dilution.Lane 1 : NIH 3T3 cell lysate not treated with ultraviolet.Lane 2 : NIH 3T3 cell lysate treated with ultraviolet.Lane 3 : NIH 3T3 cell lysate not treated with Anisomycin.Lane 4 : NIH 3T3 cell lysate treated with Anisomycin.Predicted band size : 36 kDa.Observed band size : 42 kDa .
Immunohistochemistry (Paraffin-embedded sections) - c-Jun (phospho S63) antibody [Y172]; Ab32385, at a 1/50 dilution, staining c-Jun in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.