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Anti-MAP2K1 Antibody E342
Also for MAP2K1 (NM_002755)
|A synthetic peptide corresponding to residues in the N-terminus of human MEK1 was used as an immunogen. The antibody does not cross-react with other MAP kinase kinase family member.|
|Mouse, Rat, Cow, Dog, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF, FC
||IHC-Fr: 1:400; WB: 1:1000 - 1:5000; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:100 - 1:250; FC: 1:100; IP: 1:200
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
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Western blot - MEK1 antibody [E342]; Anti-MEK1 [E342] antibody at 1/5000 dilution + A431 cell lysate.Predicted band size : 43 kDa.Observed band size : 45 kDa .
Immunohistochemistry (Paraffin-embedded sections) - MEK1 antibody [E342]; Ab32091, at a 1/100 dilution, staining MEK1 in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - MEK1 antibody [E342]; Ab32091, at a 1/100 dilution, staining MEK1 in A431 cells by Immunofluorescence.
Flow Cytometry - MEK1 antibody [E342]; Overlay histogram showing HeLa cells stained with TA300458 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.