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Home Antibody All anti-MAP2K1 antibodies

Anti-MAP2K1 Antibody E342

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300458
  • Rabbit Monoclonal Antibody against MAP2K1 (Clone E342)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400974) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)
FC(1)
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Also for MAP2K1 (NM_002755)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the N-terminus of human MEK1 was used as an immunogen. The antibody does not cross-react with other MAP kinase kinase family member.
Clone NameE342 IsotypeIgG
Species ReactivityMouse, Rat, Cow, Dog, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsIHC-Fr: 1:400; WB: 1:1000 - 1:5000; IHC-P: Use at an assay dependent concentration; ICC/IF: 1:100 - 1:250; FC: 1:100; IP: 1:200
Predicted MW Explanation 43 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Reference Data

Target NameHomo sapiens mitogen-activated protein kinase kinase 1 (MAP2K1)
Alternative NameCFC3; MAPKK1; MEK1; MKK1; PRKMK1
Database LinkNP_002746
Entrez Gene 5604 Human
Entrez Gene 26395 Mouse
Entrez Gene 170851 Rat
Entrez Gene 478347 Dog
FunctionMEK1 and MEK2 (MAPK kinase 1/2, or ERK kinase 1/2) are mitogen-activated protein kinases that stimulate MAP kinase activity, playing a role in both cell growth and differentiation (1,2). MEK itself is activated via phosphorylation at serines 217/218 and 221/222 by upstream activator kinases, including c-raf, mos and MEK kinase (3,4). MEK1 and MEK2 are activated by a wide variety of growth factors and cytokines, and also by membrane depolarization and calcium influx (5).
Related PathwayProtein KinaseDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwayOocyte meiosisVascular smooth muscle contractionDorso-ventral axis formationMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - MEK1 antibody [E342]; Anti-MEK1 [E342] antibody at 1/5000 dilution + A431 cell lysate.Predicted band size : 43 kDa.Observed band size : 45 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - MEK1 antibody [E342]; Ab32091, at a 1/100 dilution, staining MEK1 in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.
IF Image
Immunocytochemistry/ Immunofluorescence - MEK1 antibody [E342]; Ab32091, at a 1/100 dilution, staining MEK1 in A431 cells by Immunofluorescence.
FC Image
Flow Cytometry - MEK1 antibody [E342]; Overlay histogram showing HeLa cells stained with TA300458 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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