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Anti-CASP3 Antibody E83-77
Also for CASP3 (NM_032991)
|A synthetic peptide corresponding to residues following Ser29 of human caspase-3 (N-terminus of p17 subunit) was used as immunogen. This antibody only detects the active (cleaved) form of Caspase-3 and does not recognize the pro form of Caspase-3.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:500; IHC: 1:250; ICC: 1: 100-250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Does not react with Mouse, Rat
|Homo sapiens caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant beta|
|CPP32; CPP32B; SCA-1|
|Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-3 (apopain, SCA-1, Yama and CPP32) is a member of the apoptosis execution functional group of caspases, and is either partially or totally responsible for the proteolytic cleavage of many key proteins during apoptosis, such as poly (ADP-ribose) polymerase (PARP) (1,2,3). Caspase-3 is a cytosolic protein found in cells as an inactive 32 kDa proenzyme. It is activated by proteolytic cleavage into two active subunits only when cells undergo apoptosis (3).|
MAPK signaling pathway
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Western blot - active Caspase 3 antibody [Y83-77]; Lanes 1 - 2 : anti Pro Caspase 3 at 1/10000 dilution.Lanes 3 - 4 : Anti-active Caspase 3 antibody [E83-77] at 1/500 dilution.Lane 1 : Jurkat cell lysate.Lane 2 : Jurkat cell lysate + Camptothecin.Lane 3 : Jurkat cell lysate.Lane 4 : Jurkat cell lysate + Camptothecin.Predicted band size : 32 kDa.Observed band size : 17 kDa .Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Paraffin-embedded sections) - active Caspase 3 antibody [Y83-77]; Ab32042, at dilution of 1/50, staining active Caspase 3 in paraffin embedded human hepatocellular carcinoma tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - active Caspase 3 antibody [Y83-77]; Ab32042, at dilution of 1/100, staining HeLa cells by Immunofluorescence. .Left image: control. .Right image: staurosporine treated.