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Home Antibody All anti-MYC antibodies

Anti-MYC Antibody Y69

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SKU Description Amount Price Availability*  
TA300419
  • Rabbit Monoclonal Antibody against MYC (Clone Y69)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400876) , 20ug
100ul $379 Next Day
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WB(1)
IP(1)
IHC(5)
IF(3)
Assay(1)
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Also for MYC (NM_002467)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in N-terminus of human c-Myc was used as immunogen.
Clone NameY69 IsotypeIgG
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, ASSAY, IHC, IF, IP Suggested DilutionsWB: 1: 10,000; IHC: 1:50; ICC: 1:250-500; IP: 1: 150
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Reference Data

Target NameHomo sapiens v-myc avian myelocytomatosis viral oncogene homolog (MYC)
Alternative NamebHLHe39; c-Myc; MRTL; MYCC
Database LinkNP_002458
FunctionOncogene-encoded proteins c-Myc, N-Myc, and L-Myc function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors, the N-Myc gene in neuroblastomas (1), and the L-Myc gene in human small cell lung carcinomas (2). c-Myc protein is a transcription factor localized to the nucleus of the cell. It seems to be involved in activating the transcription of growth-related genes. c-Myc binds to DNA during transcription as a heterodimeric complex with Max. c-Myc is phosphorylated in vitro by p44/42 MAP kinase at Ser62 (3) and in vivo at both Thr58 and Ser62. Mutation of Thr58 and Ser62 to Ala inhibits the ability of c-Myc to activate transcription (3).
Related Pathway
Apoptosis
Cell cycle
EGFR1 Signaling Pathway
Jak-STAT signaling pathway
MAPK signaling pathway
TGF Beta Signaling Pathway
Wnt Signaling Pathway

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WB Image
Western blot - c-Myc antibody [Y69]; Anti-c-Myc antibody [Y69] at 1/10000 dilution + Jurkat cell lysate.Predicted band size : 49 kDa.Observed band size : 57 kDa .
Assay Image
Other-Anti-c-Myc antibody [Y69](TA300419); Equilibrium disassociation constant (KD)..
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-c-Myc antibody [Y69](TA300419); TA300419 showing positive staining in Colonic adenocarcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - c-Myc antibody [Y69]; Ab32072, at a dilution of 1/50, staining c-Myc in paraffin embedded human skin carcinoma tissue by Immunohistochemistry.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-c-Myc antibody [Y69](TA300419); TA300419 showing positive staining in Urinary bladder transitional carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-c-Myc antibody [Y69](TA300419); TA300419 showing positive staining in Lung adenocarcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-c-Myc antibody [Y69](TA300419); TA300419 showing positive staining in Gastric adenocarcinoma tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - c-Myc antibody [Y69]; TA300419 staining c-Myc in HEK-293 cells transfected with CACNB4-c-Myc by Immunocytochemistry/ Immunofluorescence.Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X-100 then blocked using 5% serum for 20 minutes at 25°C. Samples were then incubated with TA300419 at a 1/250 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal, used at a 1/500 dilution.
IF Image
Immunocytochemistry/ Immunofluorescence - c-Myc antibody [Y69]; Ab32072, at a dilution of 1/250, staining Hela cells by Immunoflourescence.
IF Image
Immunocytochemistry/ Immunofluorescence - c-Myc antibody [Y69]; ICC/IF image of TA300419 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was DyLight? 488 goat anti-rabbit IgG - H&L, pre-adsorbed used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IP Image
Immunoprecipitation - Anti-c-Myc antibody [Y69]; c-Myc was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5ug of Rabbit monoclonal to c-Myc [Y69] and 50ul of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40ul SDS loading buffer and incubated for 10min at 70°C; 10ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with TA300419.Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/20,000 dilution.Band: 57kDa; c-Myc [Y69]

 

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