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Anti-EIF2AK2 Antibody E120
Also for EIF2AK2 (NM_002759)
|A synthetic phospho-peptide corresponding to residues surrounding Thr446 of human PKR (Interferon-inducible RNA-dependent protein kinase) was used as immunogen. The antibody only detects PKR phosphorylated on Threonine 446.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||FC: 1:1000; IHC-P: 1:50 - 1:100; WB: 1:1000; ICC/IF: 1:100; IP: 1:10
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified
|Homo sapiens eukaryotic translation initiation factor 2-alpha kinase 2 (EIF2AK2), transcript variant 1|
|EIF2AK1; PKR; PRKR|
|Protein kinase R (PKR) is a serine/threonine kinase that is induced by interferon and activated by double-stranded RNA. Upon activation, PKR is autophosphorylated at multiple sites and phosphorylates the a-subunit of the eukaryotic initiation factor-2 eIF2 (eIF2a), inhibiting protein synthesis (1,2). PKR controls the activation of several transcription factors (1) and mediates the effects of interferon via its capacity to inhibit protein synthesis (2). It has been shown that the autophosphorylation sites Thr-446 and Thr-451 in the activation loop of Human PKR are critical for its kinase activity (3).|
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Western blot - PKR (phospho T446) antibody [E120]; All lanes : Anti-PKR (phospho T446) antibody [E120] at 1/1000 dilution.Lane 1 : Hela cell lysate.Lane 2 : Hela cell lysate + IFN + CalyculinA.Lane 3 : Hela cell lysate + Pptase.Predicted band size : 62 kDa.Observed band size : 68 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PKR (phospho T446) antibody [E120]; Immunohistochemical analysis of PKR (phospho T446) expression in normal colon tissue, using 1/50 TA300378.
Flow Cytometry - Anti-PKR (phospho T446) antibody [E120]; Overlay histogram showing HeLa cells stained with TA300378 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22Â°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22Â°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1Âµg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.