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Anti-ESR1 Antibody E91
Also for ESR1 (NM_000125)
|A synthetic phospho-peptide corresponding to residues surrounding Ser118 of human ERalpha was used as immunogen. The antibody only detects ERalpha phosphorylated on Serine 118.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1: 1,000; IHC: 1:50; ICC: 1:50-1: 100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens estrogen receptor 1 (ESR1), transcript variant 1|
|ER; Era; ESR; ESRA; ESTRR; NR3A1|
|Estrogen receptor alpha (ER alpha) is a nuclear protein and member of the steroid hormone receptor family. ER alpha possesses both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes (1). Ligand-dependent dimerization and phosphorylation on serines, 104, 106, and 118 function to regulate the transcriptional activation of ER alpha (2). The phosphorylation of the human estrogen receptor (ER) serine residue at position 118 is required for full activity of the ER activation function 1 (AF-1) (3). |
TGF Beta Signaling Pathway
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Western blot - Estrogen Receptor alpha (phospho S118) antibody [E91]; All lanes : Anti-Estrogen Receptor alpha (phospho S118) antibody [E91] at 1/1000 dilution.Lane 1 : MCF7 cell lysate (untreated).Lane 2 : MCF7 cell lysate (treated with b-Estradiol and EGF).Predicted band size : 66 kDa.Observed band size : 60 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Estrogen Receptor alpha (phospho S118) antibody [E91]; Immunohistochemical staining of (A) untreated and (B) Phosphatase-treated paraffin-embedded human breast adenocarcinoma using ab32396 at 1:50 dilution.
Immunofluorescence - Estrogen Receptor alpha (phospho S118) antibody [E91]; Immunofluorescent staining of (A) untreated and (B) Phosphatase-treated MCF-7 cells using ab32396.