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Anti-ESR1 Antibody E91
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Also for ESR1 (NM_000125)
|A synthetic phospho-peptide corresponding to residues surrounding Ser118 of human ERalpha was used as immunogen. The antibody only detects ERalpha phosphorylated on Serine 118.|
|WB, IHC, IF
||WB: 1: 1,000; IHC: 1:50; ICC: 1:50-1: 100
|50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Homo sapiens estrogen receptor 1 (ESR1), transcript variant 1|
|ER; Era; ESR; ESRA; NR3A1|
|Estrogen receptor alpha (ER alpha) is a nuclear protein and member of the steroid hormone receptor family. ER alpha possesses both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes (1). Ligand-dependent dimerization and phosphorylation on serines, 104, 106, and 118 function to regulate the transcriptional activation of ER alpha (2). The phosphorylation of the human estrogen receptor (ER) serine residue at position 118 is required for full activity of the ER activation function 1 (AF-1) (3). |
TGF Beta Signaling Pathway
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Western blot analysis on MCF7 cell lysate using anti-Phospho-ER alpha (S118); dilution 1:1,000. Cell lysates were (1) untreated, or (2) treated with b-Estradiol and EGF.
Immunohistochemical staining of (A) untreated and (B) Phosphatase-treated paraffin-embedded human breast adenocarcinoma using anti-Phospho-ER alpha (S118).
Immunofluorescent staining of (A) untreated and (B) Phosphatase-treated MCF-7 cells using anti-Phospho-ER alpha (S118)