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Home Antibody All anti-CASP3 antibodies

Anti-CASP3 Antibody E87

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300366
  • Rabbit Monoclonal Antibody against CASP3 (Clone E87)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC403215) , 20ug Explanation
100ul 325 In Stock
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WB(1)
IHC(1)
FC(1)
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Also for CASP3 (NM_032991)
cDNA Clone shRNA/siRNA Protein/Lysate Antibody Assay

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in p17 subunit of human Caspase-3 was used as immunogen. The antibody should recognize both pro-form (35kDa) and p17 cleaved-form of Caspase-3.
Clone NameE87 IsotypeIgG
Species ReactivityRat, Human (Does not react with: Mouse) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:5000; IHC-P: 1:25 - 1:50; ICC: 1:25; FC: 1:1000; IP: 1:10
Predicted MW Explanation 32 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Does not react with Mouse

Reference Data

Target NameHomo sapiens caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant beta
Alternative NameCPP32; CPP32B; SCA-1
Database LinkNP_116786
Entrez Gene 836 Human
Entrez Gene 25402 Rat
FunctionCaspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-3 (apopain, SCA-1, Yama and CPP32) is a member of the apoptosis execution functional group of caspases, and is either partially or totally responsible for the proteolytic cleavage of many key proteins during apoptosis, such as poly (ADP-ribose) polymerase (PARP) (1,2,3). Caspase-3 is a cytosolic protein found in cells as an inactive 32 kDa proenzyme. It is activated by proteolytic cleavage into two active subunits only when cells undergo apoptosis (3).
Related PathwayES Cell Differentiation/IPSProteaseDruggable Genome MAPK signaling pathwayp53 signaling pathwayApoptosisNatural killer cell mediated cytotoxicityAlzheimer's diseaseParkinson's diseaseMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Caspase 3 antibody [E87]; All lanes : Anti-Caspase 3 [E87] antibody at 1/5000 dilution.Lane 1 : Untreated Jurkat cell lysate.Lane 2 : Jurkat cell lysate treated with Camptothecin.Predicted band size : 32 kDa.Observed band size : 35 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Caspase 3 antibody [E87]; Ab32351, at a 1/25 dilution, staining Capase 3 in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.
FC Image
Flow Cytometry - Anti-Caspase 3 antibody [E87]; Overlay histogram showing Jurkat cells stained with TA300366 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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