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Home Antibody All anti-CASP3 antibodies

Anti-CASP3 Antibody E87

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Specifications Citations (1) Related Products Product Documents
SKU Description Amount Price Availability*  
TA300366 Rabbit Monoclonal Antibody against CASP3 (Clone E87) 100ul $325 In Stock
LC403215 CASP3 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(1)
IHC(1)
FC(1)
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Also for CASP3 (NM_032991)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Assay

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in p17 subunit of human Caspase-3 was used as immunogen. The antibody should recognize both pro-form (35kDa) and p17 cleaved-form of Caspase-3.
Clone NameE87 IsotypeIgG
Species ReactivityRat, Human (Does not react with: Mouse) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:5000; IHC-P: 1:25 - 1:50; ICC: 1:25; FC: 1:1000; IP: 1:10
Predicted MW Explanation 32 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Immunogen affinity purified
Note Does not react with Mouse

Reference Data

Target NameHomo sapiens caspase 3, apoptosis-related cysteine peptidase (CASP3), transcript variant beta
Alternative NameCPP32; CPP32B; SCA-1
Database LinkNP_116786
Entrez Gene 836 Human
Entrez Gene 25402 Rat
FunctionCaspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-3 (apopain, SCA-1, Yama and CPP32) is a member of the apoptosis execution functional group of caspases, and is either partially or totally responsible for the proteolytic cleavage of many key proteins during apoptosis, such as poly (ADP-ribose) polymerase (PARP) (1,2,3). Caspase-3 is a cytosolic protein found in cells as an inactive 32 kDa proenzyme. It is activated by proteolytic cleavage into two active subunits only when cells undergo apoptosis (3).
Related PathwayES Cell Differentiation/IPSProteaseDruggable Genome MAPK signaling pathwayp53 signaling pathwayApoptosisNatural killer cell mediated cytotoxicityAlzheimer's diseaseParkinson's diseaseMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Caspase 3 antibody [E87]; All lanes : Anti-Caspase 3 [E87] antibody at 1/5000 dilution.Lane 1 : Untreated Jurkat cell lysate.Lane 2 : Jurkat cell lysate treated with Camptothecin.Predicted band size : 32 kDa.Observed band size : 35 kDa .
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Caspase 3 antibody [E87]; Ab32351, at a 1/25 dilution, staining Capase 3 in paraffin embedded human cervical carcinoma tissue by Immunohistochemistry.
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Flow Cytometry - Anti-Caspase 3 antibody; Overlay histogram showing Jurkat cells stained with TA300366 (red line). The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1:2000. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control.
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