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Anti-YWHAB Antibody Y62
Also for YWHAB (NM_139323)
|A synthetic peptide corresponding to C-terminal residues of human 14-3-3 ß/a was used as immunogen. Predicted to cross-react with rat and bovine, based on sequence homology.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000; IHC-P: Use at an assay dependent dilution; ICC: 1:500; IP: 1:40; FC: 1:40
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Homo sapiens tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide (YWHAB), transcript variant 2|
|GW128; HS1; KCIP-1; YWHAA|
|The 14-3-3 proteins are a family of proteins involved in the regulation of apoptosis, mitogenic signaling and cell-cycle checkpoints (1). The 14-3-3 proteins are thought to be key regulators of signal transduction events mediated through their binding to serine-phosphorylated proteins (2,3). Through binding Bad, 14-3-3 prevents apoptosis by sequestering Bad to the cytosol (3).|
EGFR1 Signaling Pathway
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Western blot - 14-3-3 alpha + beta antibody [Y62]; Anti-14-3-3 alpha + beta antibody [Y62] at 1/1000 dilution + Hela cell lysate.Predicted band size : 30 kDa.Observed band size : 28 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 14-3-3 alpha + beta antibody [Y62]; Ab32560, at a 1/500 dilution, staining 14-3-3 alpha + beta in paraffin embedded human breast tissue by Immunohistochemistry.