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Home Antibody All anti-LAT antibodies

Anti-LAT Antibody E225

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300326
  • Rabbit Monoclonal Antibody against LAT (Clone E225)
100ul 325 3-7 Days
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WB(1)
IHC(1)

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Tyr191 of human LAT was used as immunogen. The antibody only detects LAT phosphorylated on Tyrosine 191.
Clone NameE225 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:2,000; IHC: 1:50; ICC: 1:50; IP: 1: 10
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Protein A purified

Reference Data

Target NameHomo sapiens linker for activation of T cells (LAT), transcript variant 1
Alternative NameLAT1; pp36
Database LinkNP_055202
Entrez Gene 27040 Human
Entrez Gene 16797 Mouse
Entrez Gene 81511 Rat
FunctionLAT (linker for activation of T-cells) is involved in the T-cell antigen receptor (TCR) signal transduction pathway, and may play an important role downstream in activating protein tyrosine kinases (1). LAT is phosphorylated by ZAP-70/Syk protein tyrosine kinases leading to recruitment of multiple signaling molecules (2). It has been found that Tyr191 and Tyr171 are required for T-cell activation and Tyr132 is required for the activation of PLCgamma1 and Ras signaling pathways, respectively. Tyr226 and Tyr191 are required for Vav binding, whereas Tyr171 and Tyr132 are necessary for association and activation of phosphoinositide 3-kinase activity and PLCgamma1 (3).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-LAT (phospho Y191) antibody [E225]; All lanes : Anti-LAT (phospho Y191) antibody [E225] at 1/1000 dilution.Lane 1 : Untreated Jurkat cell lysate.Lane 2 : Jurkat cell lysate treated with anti-CD3.Predicted band size : 28 kDa.Observed band size : 38 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - LAT (phospho) antibody [E205]; TA300326, at a 1/50 dilution, staining human tonsils by immunohistochemistry, Paraffin embedded tissue.

 

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