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Anti-LAT Antibody E225
|A synthetic phospho-peptide corresponding to residues surrounding Tyr191 of human LAT was used as immunogen. The antibody only detects LAT phosphorylated on Tyrosine 191.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:2,000; IHC: 1:50; ICC: 1:50; IP: 1: 10
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified (Protein A or G Sepharose)
|Homo sapiens linker for activation of T cells (LAT), transcript variant 1|
|LAT (linker for activation of T-cells) is involved in the T-cell antigen receptor (TCR) signal transduction pathway, and may play an important role downstream in activating protein tyrosine kinases (1). LAT is phosphorylated by ZAP-70/Syk protein tyrosine kinases leading to recruitment of multiple signaling molecules (2). It has been found that Tyr191 and Tyr171 are required for T-cell activation and Tyr132 is required for the activation of PLCgamma1 and Ras signaling pathways, respectively. Tyr226 and Tyr191 are required for Vav binding, whereas Tyr171 and Tyr132 are necessary for association and activation of phosphoinositide 3-kinase activity and PLCgamma1 (3).|
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Western blot - Anti-LAT (phospho Y191) antibody [E225]; All lanes : Anti-LAT (phospho Y191) antibody [E225] at 1/1000 dilution.Lane 1 : Untreated Jurkat cell lysate.Lane 2 : Jurkat cell lysate treated with anti-CD3.Predicted band size : 28 kDa.Observed band size : 38 kDa .
Immunohistochemistry (Paraffin-embedded sections) - LAT (phospho) antibody [E205]; ab32434, at a 1/50 dilution, staining human tonsils by immunohistochemistry, Paraffin embedded tissue.