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Anti-HDAC2 Antibody Y461


Specifications Citations (1) Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA300044 Histone Deacetylase 2 (C-term) Rabbit Monoclonal Antibody 100ul $325 In Stock
LC419878 HDAC2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the C-term of human Histone Deacetylase 2 was used as immunogen.
Clone NameY461 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:2000; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:250 - 1:500; FC: 1:60 - 1:100; IP: 1:60; IHC-Fr: 1:500
Predicted MW Explanation 55 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Reference Data

Target NameHomo sapiens histone deacetylase 2 (HDAC2), transcript variant 1
Alternative NameHD2; RPD3; YAF1
Database LinkNP_001518
Entrez Gene 3066 Human
Entrez Gene 15182 Mouse
Entrez Gene 84577 Rat
FunctionHistone deacetylase 2 (HDAC2) belongs to the histone deacetylase family type 1 which is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (1). HDAC2 represses transcription and binds transcription factor YY1 to form a large complex (2). It also forms transcriptional repressor complexes by associating with MAD, SIN3, N-CoR and DNMT1 (3-4). Similar to HDAC1 and HDAC3, HDAC2 does not possess a repression domain, but preferentially associates with HDAC1. HDAC2 with HDAC1 is also implicated in a complex of multiproteins that functions through modifying chromatin structure to keep genes silent (5).
Related PathwayStem cell - PluripotencyTranscription FactorsDruggable Genome Cell cycleNotch signaling pathwayHuntington's diseasePathways in cancerChronic myeloid leukemia

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - HDAC2 antibody [Y461]; Anti-HDAC2 antibody [Y461] at 1/2000 dilution + K562 cell lysate.Predicted band size : 55 kDa.Observed band size : 70 kDa .

IHC Image
Immunohistochemistry (Paraffin-embedded sections) - HDAC2 antibody [Y461]; Immunohistochemical analysis of HDAC2 expression in paraffin embedded human breast carcinoma tissue section, using 1/250 TA300044.

IHC Image
TA300044 staining HDAC2 in Rat spinal cord tissue sections by IHC (IHC-Fr - frozen sections). Samples were incubated with primary antibody (1/500 in PBS + 0.2% TritonX + 1% BSA) for 16 hours at 4°C. An Alexa Fluor?488-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Antigen unmasking with sodium citrate buffer (10mM sodium citrate, 0.05% Tween 20, ph6) was necessary to obtain a good signal. The sections were counterstained with DAPI.

IHC Image
Figure from citation: Immunohistochemistry of HDAC2 protein level by using anti-HDAC2 antibody in AOM-induced mouse colonic preneoplastic lesions (ACF). Dilution: 1:100 View Citation

IF Image
Immunocytochemistry/ Immunofluorescence - HDAC2 antibody [Y461]; Immunofluorescent analysis of HDAC expression in HeLa cells, using 1/250 TA300044.

FC Image
Flow Cytometry-HDAC2 antibody(TA300044); Overlay histogram showing HeLa cells stained with TA300044 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit monoclonal IgG (1ug/1x10^6 cells) used under the same conditions. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.


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