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Anti-SYP Antibody YE269
|A synthetic peptide corresponding to residues in C-terminus (cytoplasmic domain) of human Synaptophysin was used as immunogen. Predicted to cross-react with bovine, based on sequence homology.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, IF
||WB: 1:10000 - 1:50000; ICC/IF: 1:250; IHC-P: 1:250 - 1:500
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens synaptophysin (SYP)|
Entrez Gene 6855 Human
Entrez Gene 20977 Mouse
Entrez Gene 24804 Rat
|Synaptophysin (SypI), also referred as p38 (1), constitutSyp I contains four transmembrane regions and can be phosphorylated on its cytoplasmic C-terminus by both serine/threonine and tyrosine kinases (2). In addition to syp I, synaptic vesicles contain a highly homologous membrane protein named synaptoporin or synaptophysin II (Syp II) (3). While Syp I is ubiquitously expressed and present in virtually all synapses whereas Syp II is expressed at high levels only in selected neurons (4). es approximately 7% of the total vesicle protein and is selectively associated with small synaptic vesicles (SSVs)|
|Secreted ProteinTransmembrane |
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Western blot - Synaptophysin antibody [YE269]; Anti-Synaptophysin antibody [YE269] at 1/10000 dilution + PC12 cell lysate.Predicted band size : 34 kDa.Observed band size : 34 kDa.Additional bands at : 21 kDa,22 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269]; TA300431 showing positive staining in Medulloblastoma tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269]; TA300431 showing positive staining in Lung neuroendocrine tumor tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synaptophysin antibody [YE269]; TA300431 showing positive staining in normal pancreas tissue.
Immunofluorescence - Synaptophysin antibody [YE269]; Ab32127, at a dilution of 1/250, staining Synaptophysin in PC12 cells by Immunofluorescence.