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Anti-SUFU Antibody EPR1206Y
Also for SUFU (NM_016169)
|A synthetic peptide corresponding to peptides on the N-terminus o f human SuFu was used as an immunogen.|
||Tissue culture supernatant
||Lot dependent; please refer to CoA along with shipment
|WB, IHC, FC
||WB: 1:1000 - 1:2000; IP: 1:50; IHC-P: 1:100; FC: 1:100
|Does not react with Mouse, Rat. Is unsuitable for ICC/IF.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for ICC/IF.
|Homo sapiens suppressor of fused homolog (Drosophila) (SUFU), transcript variant 1|
|PRO1280; SUFUH; SUFUXL|
|The Hedgehog (Hh) signaling pathway has conserved roles in development of species ranging from Drosophila to humans. Human supressor of fused Su(Fu) acts as a negative regulator in the hedgehog signaling (SHH) pathway by down-regulating GLI1-mediated transactivation of target genes (1). Su(fu) was found to repress activity of the zinc-finger transcription factor Gli, which mediates Hedgehog signaling in vertebrates, and to physically interact with Gli, Gli2 and Gli3 (2). The sonic hedgehog signaling pathway directs the embryonic development of diverse organisms and is disrupted in a variety of malignancies. Several of mutations encode truncated proteins that are unable to export the GLI transcription factor from nucleus to cytoplasm, resulting in the activation of SHH signaling. SUFU is a newly identified tumor-suppressor gene that predisposes individuals to medulloblastoma by modulating the SHH signaling pathway through a newly identified mechanism (3). |
Hedgehog signaling pathway
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Western blot - Suppressor of Fused antibody [EPR1206Y]; All lanes : Anti-Suppressor of Fused antibody [EPR1206Y] at 1/2000 dilution.Lane 1 : Hela cell lysate.Lane 2 : Human kidney cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 54 kDa.Observed band size : 54 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Suppressor of Fused antibody [EPR1206Y]; TA301261 at 1/100 dilution staining Suppressor of Fused in human testis tissue, using a HRP/AP polymerized secondary antibody.
Flow Cytometry - Anti-Suppressor of Fused antibody [EPR1206Y]; Overlay histogram showing HEK293 cells stained with TA301261 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22Â°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22Â°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1Âµg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.