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Anti-SQSTM1 TRUEMAB Antibody clone 5A2
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for SQSTM1 (NM_003900)
|Full length human recombinant protein of human SQSTM1 (NP_003891) produced in HEK293T cell. |
|Human , Monkey
|WB, IHC, IF, FC
|| WB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100,
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens sequestosome 1 (SQSTM1), transcript variant 1|
|A170; OSIL; p60; p62; p62B; PDB3; ZIP3|
|This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SQSTM1 (RC203214, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SQSTM1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SQSTM1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502131)
Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-SQSTM1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502131)
Anti-SQSTM1 mouse monoclonal antibody (TA502131) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SQSTM1(RC203214).
HEK293T cells transfected with either RC203214 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SQSTM1 antibody(TA502131), and then analyzed by flow cytometry.