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Home Antibody All anti-SOX2 antibodies

Anti-SOX2 Antibody EPR3131

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307386
  • Rabbit monoclonal antibody against SOX2 (clone EPR3131 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401083) , 20ug
100ul 325 In Stock
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WB(1)
IHC(9)
ELISA(1)
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Also for SOX2 (NM_003106)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human SOX2 was used as an immunogen.
Clone NameEPR3131 IsotypeTissue culture supernatant
Species ReactivityHuman ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ELISA, IHC Suggested DilutionsWB: 1:1000 - 1:10000; ICC: 1:50 - 1:100; Sandwich ELISA: Use a concentration of 0.5 ug/ml; IHC-P: 1:100 - 1:250; IHC - Wholemount: Use at an assay dependent concentration; ICC/IF: Use at an assay dependent concentration
BufferDoes not react with Rat. Is unsuitable for IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens SRY (sex determining region Y)-box 2 (SOX2)
Alternative NameANOP3; MCOPS3
Database LinkNP_003097
FunctionSOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. It is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach (1). SOX2 is necessary for regulating multiple transcription factors that affect Oct3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct3/4 expression (2).
Related Pathway
Wnt Signaling Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - SOX2 antibody [EPR3131]; All lanes : Anti-SOX2 [EPR3131] antibody at 1/5000 dilution.Lane 1 : NCCIT cell lysate.Lane 2 : MCF-7 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 34 kDa.
ELISA Image
Sandwich ELISA - SOX2 antibody [EPR3131]; Standard Curve for SOX2 (Analyte: ; dilution range 1pg/ml to 1ug/ml using Capture Antibody at 0.2ug/ml and Detector Antibody at 0.5ug/ml.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SOX2 antibody [EPR3131]; TA307386 at 1/100 dilution staining SOX2 in (1) glioma tissue, (2) Human adult brain tissue & (3) Human fetal brain tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-SOX2 antibody [EPR3131](TA307386); TA307386 showing negative staining in Seminoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-SOX2 antibody [EPR3131](TA307386); TA307386 showing positive staining in Breast carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131]; TA307386 showing negative staining in Normal lung tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-SOX2 antibody [EPR3131](TA307386); TA307386 showing positive staining in Fetal stomach tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131]; TA307386 showing positive staining in Fetal lung tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131]; TA307386 showing positive staining in Embryonal carcinoma tissue.
IHC Image
IHC - Wholemount - Anti-SOX2 antibody [EPR3131]; IHC-Wholemount image of anti-Sox2 staining on a mouse blastocyst. This blastocyst was stained with anti-Sox2 antibody (pink) at a 1:200 dilution for 48 hours at 4oC. Nuclei stained with DAPI (grey).
IHC Image
IHC - Wholemount - Anti-SOX2 antibody [EPR3131]; TA307386 at 1/200 dilution staining SOX2 inIHC wholemount ofLeucoraja erinacea embryo.Sample was incubated with antibody for 48hours at 4°C in 10% fetal calf serum in PBT.Detection: DAB

 

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