OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-SOX2 antibodies

Anti-SOX2 Antibody EPR3131

div

Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA307386 Rabbit monoclonal antibody against SOX2 (clone EPR3131 ) 100ul $325 In Stock
LC401083 SOX2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
Add to Shopping Cart
spacer
WB(1)
IHC(9)
ELISA(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human SOX2 was used as an immunogen.
Clone NameEPR3131 IsotypeIgG
Species ReactivityMouse, Human, Leucoraja erinacea (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, ELISA, IHC Suggested DilutionsWB: 1:1000 - 1:10000; ICC: 1:50 - 1:100; Sandwich ELISA: Use a concentration of 0.5 ug/ml; IHC-P: 1:100 - 1:250; IHC - Wholemount: Use at an assay dependent concentration; ICC/IF: Use at an assay dependent concentration
BufferpH: 7.20; Preservative: 0.01% Sodium azide; Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Purification Protein A purified
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens SRY (sex determining region Y)-box 2 (SOX2)
Alternative NameANOP3; MCOPS3
Database LinkNP_003097
Entrez Gene 6657 Human
Entrez Gene 20674 Mouse
FunctionSOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. It is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach (1). SOX2 is necessary for regulating multiple transcription factors that affect Oct3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct3/4 expression (2).
Related PathwayES Cell Differentiation/IPSAdult stem cellsCancer stem cellsEmbryonic stem cellsInduced pluripotent stem cellsTranscription Factors

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - SOX2 antibody [EPR3131]; All lanes : Anti-SOX2 [EPR3131] antibody at 1/5000 dilution.Lane 1 : NCCIT cell lysate.Lane 2 : MCF-7 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 34 kDa.
spacer
Sandwich ELISA - SOX2 antibody [EPR3131]; Standard Curve for SOX2 (Analyte: ; dilution range 1pg/ml to 1ug/ml using Capture Antibody at 0.2ug/ml and Detector Antibody at 0.5ug/ml.
spacer
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SOX2 antibody [EPR3131]; TA307386 at 1/100 dilution staining SOX2 in (1) glioma tissue, (2) Human adult brain tissue & (3) Human fetal brain tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
spacer
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-SOX2 antibody [EPR3131](TA307386); TA307386 showing negative staining in Seminoma tissue.
spacer
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-SOX2 antibody [EPR3131](TA307386); TA307386 showing positive staining in Breast carcinoma tissue.
spacer
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131]; TA307386 showing negative staining in Normal lung tissue.
spacer
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-SOX2 antibody [EPR3131](TA307386); TA307386 showing positive staining in Fetal stomach tissue.
spacer
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131]; TA307386 showing positive staining in Fetal lung tissue.
spacer
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody [EPR3131]; TA307386 showing positive staining in Embryonal carcinoma tissue.
spacer
IHC - Wholemount - Anti-SOX2 antibody [EPR3131]; IHC-Wholemount image of anti-Sox2 staining on a mouse blastocyst. This blastocyst was stained with anti-Sox2 antibody (pink) at a 1:200 dilution for 48 hours at 4oC. Nuclei stained with DAPI (grey).
spacer
IHC - Wholemount - Anti-SOX2 antibody [EPR3131]; TA307386 at 1/200 dilution staining SOX2 inIHC wholemount ofLeucoraja erinacea embryo.Sample was incubated with antibody for 48hours at 4°C in 10% fetal calf serum in PBT.Detection: DAB
spacer

 

spacer
Inc 5000 Healthcare Company
All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title

Notice to Non-US Customers

ExclaimationWeb price and delivery time are for direct sales only.

Non-US customers are encouraged to contact our dedicated distributor network for quotes and streamlined ordering/delivery support.

Acknowledge