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Anti-SMAD3 Antibody EP823Y
Also for SMAD3 (NM_005902)
|A synthetic phospho-specific peptide corresponding to residues surrounding Ser423 and Ser425 of human Smad3 was used as an immunogen. The antibody only detects Smad3 phosphorylated on Serine 423 and Serine 425.|
||Tissue culture supernatant
|Human, Mouse(WB only)
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||WB: 1:2000; ICC/IF: 1:100 - 1:250; ELISA: Use at an assay dependent concentration; IHC-Fr: Use at an assay dependent concentration; IHC-P: 1:100 - 1:250
|Does not react with Rat. Is unsuitable for Flow Cyt or IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt or IP.
|Homo sapiens SMAD family member 3 (SMAD3), transcript variant 1|
|HSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3|
|Smad proteins belong to a group of intracellular signal transducers and downstream effectors of TGF-ß/BMP Signaling. The family consists of nine members that are highly conserved in the N- and C-terminal regions (1). Smad3 is a key component in intracellular signaling of transforming growth factor beta (TGF-ß), an inhibitor for tumor cell proliferation (2). Smad3 is activated by activin/TGF-ß receptors, which form a heteromeric complex with Smad4, translocating to the nucleus and regulating the expression of TGF- ß target genes. Interaction of Smad3 with Akt, can initiate TGF- ß induced apoptosis and cell cycle arrest (3). Smad3 expression may be critical in tumor suppression in early stages of gastric carcinogenesis |
Delta-Notch Signaling Pathway
EGFR1 Signaling Pathway
TGF Beta Signaling Pathway
Wnt Signaling Pathway
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Western blot - Anti-Smad3 (phospho S423 + S425) [EP823Y] antibody; .Predicted band size : 48 kDa.
Western blot - Smad3 antibody [EP823Y]; All lanes : Anti-Smad3 (phospho S423 + S425) [EP823Y] antibody at 1/2000 dilution.Lane 1 : (A) HL-60 cell lysates at 10ug untreated.Lane 2 : (B) HL-60 cell lysates at 10ug treated with TGF.Predicted band size : 48 kDa.Observed band size : 55 kDa .Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.
Western blot - Smad3 (phospho S423 + S425) antibody [EP823Y]; All lanes : Anti-Smad3 (phospho S423 + S425) [EP823Y] antibody at 1/1000 dilution.Lane 1 : Lysate prepared from untreated human A549 cells.Lane 2 : Lysate prepared from untreated human A549 cells for 30min.Lane 3 : Lysate prepared from TGF-?1 cells at 10ng/ml for 30min.Lane 4 : Lysate prepared from TNF-a cells at 20ng/ml for 30min.Lane 5 : Lysate prepared from TGF-?1 and TNF-a cells at above doses for 30min.Lane 6 : Blank DMEM media.Lysates/proteins at 20 µg per lane.Secondary.Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) .developed using the ECL technique.Performed under reducing conditions.Predicted band size : 48 kDa.Observed band size : 48 kDa.Exposure time : 1 hourThis image is a courtesy of Aaron Gardner
Immunohistochemistry - Smad3 (phospho S423 + S425) antibody [EP823Y]; Immunohistochemical analysis of Smad3 in paraffin embedded human liver carcinoma tissue using ab52903 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Smad3 antibody [EP823Y]; Immunofluorescent staining of Smad3 in HeLa cells using ab52903 at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Smad3 (phospho S423 + S425) antibody [EP823Y]; ab52903 stainingSmad3 (phospho S423 + S425) inhuman TII Pneumocyte A549c ells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde andpermeabilized with 0.1%Triton x100before blocking with 3% BSA for 1 hour at RT. Samples were incubated with primary antibody (1/200: in 3% BSA in 1x PBST) for24 hours at 4°C. A TRITC-conjugatedgoat polyclonal torabbit IgG was used as secondary antibody at 1/200 dilution.