Also for SIM2 (NM_011377)
|Peptide with sequence C-SDLLYTPSYS, from the internal region of the protein sequence according to NP_005060.1; NP_033664.2.|
|Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20C. Minimize freezing and thawing.|
|Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
|Mus musculus single-minded homolog 2 (Drosophila) (Sim2)|
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(0.5ug/ml) staining of fetal Mouse kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.