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Anti-SHC1 TRUEMAB Antibody clone 1H8
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for SHC1 (NM_003029)
|Full length human recombinant protein of human SHC1 (NP_003020) produced in HEK293T cell.|
|WB, IHC, IF
||WB 1:2000, IHC 1:50, IF 1:100,
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.16% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens SHC (Src homology 2 domain containing) transforming protein 1 (SHC1), transcript variant 2|
Entrez Gene 6464 Human
|Signaling adapter that couples activated growth factor receptors to signaling pathway. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span |
|Druggable Genome ErbB signaling pathwayChemokine signaling pathwayFocal adhesionNatural killer cell mediated cytotoxicityNeurotrophin signaling pathwayInsulin signaling pathwayMore Pathways >> |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SHC1 (RC204362, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SHC1.
Immunohistochemical staining of paraffin-embedded colon tissue using anti-SHC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501075, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-SHC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501075, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-SHC1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501075, Dilution 1:50)
Anti-SHC1 mouse monoclonal antibody (TA501075) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SHC1(RC204362).