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Anti-SH2B3 TRUEMAB Antibody clone 2B11
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for SH2B3 (NM_005475)
|Full length human recombinant protein of human SH2B3 (NP_005466) produced in HEK293T cell.|
|WB, IF, FC
||WB 1:500, IF 1:100, FLOW 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens SH2B adaptor protein 3 (SH2B3), transcript variant 1|
Entrez Gene 10019 Human
|This gene encodes a member of the SH2B adaptor family of proteins, which are involved in a range of signaling activities by growth factor and cytokine receptors. The encoded protein is a key negative regulator of cytokine signaling and plays a critical role in hematopoiesis. Mutations in this gene have been associated with susceptibility to celiac disease type 13 and susceptibility to insulin-dependent diabetes mellitus. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SH2B3 (RC218359, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SH2B3.
Anti-SH2B3 mouse monoclonal antibody (TA502748) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SH2B3(RC218359 ).
HEK293T cells transfected with either RC218359 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-SH2B3 antibody(TA502748), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-SH2B3 antibody(TA502748),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-SH2B3 antibody(TA502748),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).