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Anti-SF3A1 Antibody EPR7666
Also for SF3A1 (NM_001005409)
|A synthetic peptide corresponding to residues in human SF3A1 was used as an immunogen.|
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000 - 1:10000; IHC-P: 1:50 - 1:100
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Is unsuitable for Flow Cyt,ICC or IP.
|Homo sapiens splicing factor 3a, subunit 1, 120kDa (SF3A1), transcript variant 2|
|PRP21; PRPF21; SAP114; SF3A120|
Entrez Gene 10291 Human
|SF3A1 is subunit 1 of the splicing factor 3a protein complex. The splicing factor 3a heterotrimer includes subunits 1, 2 and 3 and is necessary for the in vitro conversion of 15S U2 snRNP into an active 17S particle that performs pre-mRNA splicing. SF3A1 belongs to the SURP protein family; named for the SURP (also called SWAP or Suppressor-of-White-APricot) motifs that are thought to mediate RNA binding. It has tandemly repeated SURP motifs in its amino-terminal half, while its carboxy-terminal half contains a proline-rich region and a ubiquitin-like domain. Binding studies with truncated SF3A1 derivatives demonstrated that the two SURP motifs are necessary for binding to subunit 3, while contacts with subunit 2 may occur through sequences carboxy-terminal to the SURP motifs (1). |
|Druggable Genome Spliceosome|
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Western blot - Anti-SF3A1 antibody [EPR7666]; All lanes : Anti-SF3A1 antibody [EPR7666] at 1/1000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : Lymph node lysate.Lane 3 : K562 cell lysate .Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 89 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A1 antibody [EPR7666]; TA310843, at a 1/50 dilution, staining SF3A1 in paraffin embedded Human breast tissue by Immunohistochemistry.