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Also for SERPINB2 (NM_001143818)
|This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal area of human PAI-2 protein.|
||ELISA: 1:100,000 – 150,000, WB: 1:500 – 1:2,000
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|Extracellular plasminogen activator inhibitor type-2 (also known as PAI-2, Placental plasminogen activator inhibitor; Monocyte Arg-serpin; Urokinase inhibitor; SERPINB2 serpin peptidase inhibitor, clade B (ovalbumin), member 2) is a coagulation factor and a potent inhibitor of urokinase-type plasminogen activator (u-PA) and also acts as a multifunctional protein. It is present in most cells, especially monocytes and macrophages. PAI-2 exists in two forms, a 60-kDa, secreted, extracellular, glycosylated form and a 43-kDa intracellular form. It is a multifunctional protein that plays a role in cell differentiation, in prevention of programmed cell death, in the regulation of cell proliferation, in the inhibition of microbial proteinases and in the protection against stromal degradation. High levels of the PAI-2 protein are associated with a good prognosis in breast cancer, small cell lung, ovarian cancer, and inhibition of metastasis. PAI-2 also plays a role in inflammation on the surface of the eye. PAI-2 may cooperate with pRb2/p130 in modulating PAI-2 gene expression by chromatin remodeling.
|Homo sapiens serpin peptidase inhibitor, clade B (ovalbumin), member 2 (SERPINB2), transcript variant 1|
|HsT1201; PAI; PAI-2; PAI2; PLANH2|
Senescence and Autophagy
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Western blot using affinity purified anti-PAI-2 antibody shows detection of endogenous PAI-2 in 50 µg of HeLa whole cell lysates. The band at ~42 kDa (arrowhead) corresponds to PAI-2. Faint non-specific bands are also noted at lower and higher molecular weights positions. Primary antibody was used at a 1:500 dilution in 5% BLOTTO in PBS reacted overnight at 4°C. The membrane was washed and reacted with a 1:5,000 dilution of HRP- conjugated Gt-a-Rabbit IgG (p/n 611-103-122) for 45 min at room temperature with ECL used for detection. Molecular weight estimation was made by comparison to prestained MW markers. Personal communication, Luca D’Agostino, Temple University, Philadelphia, PA