Home Antibody All anti-SERPINA1 antibodies
Also for SERPINA1 (NM_000295)
|a1-Anti-Trypsin [Human Plasma]|
||Lot dependent; please refer to CoA along with shipment
||ELISA: 1:20,000 - 1:100,000, WB: 1:2,000 - 1:10,000
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
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Anti-Trypsin Polyclonal Antibody-Multiplex Fluorescent WB. Lane 1: molecular weight. Lane 2: Alpha-1-Anti-Trypsin (red), Rabbit anti-Transferrin, and Mouse-a-GST were used to detect target proteins under reducing conditions in albumin depleted human serum. Primary antibody: Each primary antibody at 1:1000 . Secondary antibody: DyLight549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000.
Western Blot of Goat anti Alpha-1anti-Trypsin antibody. Lane 1: Alpha-1anti-Trypsin under reducing conditions. Lane 2: none. Load: 1 µg per lane. Primary antibody: Alpha-1anti-Trypsin antibody at 1:3000 for overnight at 4°C. Secondary antibody: Dylight 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 45 min at RT. Block: MB-070 overnight at 4°C. Predicted/Observed size: 55kDa for a-1 Anti-Trypsin. Other band(s): none.
WB of goat anti-Alpha 1 anti trypsin antibody. Lane 1: molecular weight. Lane 2: Human transferrin (green); Alpha 1 anti trypsin (red); and Human IgG (Blue) in a multiplex fluorescent WB of human serum. Load: 35 µg per lane. Primary antibody: Each primary antibody was diluted to 1:1000 for overnight at 4°C.