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Anti-SCRIB Antibody EPR4140(2)
Also for SCRIB (NM_015356)
|A synthetic peptide corresponding to residues near the C-terminus of human Scribble was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; ICC: 1:100 - 1:250; FC: 1:100 - 1:500
|Does not react with Mouse, Rat. Is unsuitable for IHC-P or IP.|
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA (Protein A or G Sepharose)
|Is unsuitable for IHC-P or IP.
|Homo sapiens scribbled planar cell polarity protein (SCRIB), transcript variant 2|
|CRIB1; SCRB1; SCRIB1; Vartul|
|Scribble is a cytoplasmic multimodular scaffold protein targeted to epithelial adherens junctions and neuronal presynaptic compartments. Scribble and its orthologs in vertebrates and invertebrates participate in cell polarization (1). It may regulate cell invasion through MAPK signaling. Scribble functions as an activator of Rac GTPase activity (2). Deficiency in Scribble impairs many aspects of cell polarity and cell movement (1).|
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Western blot - Anti-SCRIBBLE antibody [EPR4140(2)]; All lanes : Anti-SCRIBBLE antibody [EPR4140(2)] at 1/1000 dilution.Lane 1 : 293T cell lysate.Lane 2 : HeLa cell lysate.Lane 3 : MCF7 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-Rabbit HRP at 1/2000 dilution.Predicted band size : 175 kDa.
Flow Cytometry - Anti-SCRIBBLE antibody [EPR4140(2)]; ab125080 at 1/100, staining SCRIBBLE in permeabilized 293T cells by flow cytometry (red), compared with a negative control Rabbit IgG antibody (green).