Home Antibody All anti-SAE1 antibodies
Also for SAE1 (NM_005500)
|This SAE1 (AOS1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300-329 amino acids from the C-terminal region of human SAE1 (AOS1).|
|Human, Mouse (Predicted: Rat, Bovine)
||WB: 1:1000, IHC: 1:50~100
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.|
|This antibody is purified through a protein A column, followed by peptide affinity purification.
|Homo sapiens SUMO1 activating enzyme subunit 1 (SAE1), transcript variant 1|
|AOS1; HSPC140; SUA1; UBLE1A|
Entrez Gene 10055 Human
Entrez Gene 56459 Mouse
Entrez Gene 308384 Rat
|The dimeric enzyme AOS1 acts as a an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.|
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The AOS1 C-term Pab (Cat. #TA302053) is used in Western blot to detect AOS1 in mouse heart tissue lysate.
Western blot analysis of AOS1 (arrow) using rabbit polyclonal AOS1 Antibody (C-term) (Cat.#TA302053). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AOS1 gene (Lane 2) (Origene Technologies).
AOS1 Antibody (V315) (Cat. #TA302053) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the AOS1 antibody detected the AOS1 protein (arrow).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.