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Also for SAE1 (NM_005500)
|This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human AOS1.|
||WB: 1: 100~500; IHC: 1:50~100; ELISA: 1: 1,000;
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.|
|Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. (Protein A or G Sepharose)
|Homo sapiens SUMO1 activating enzyme subunit 1 (SAE1), transcript variant 1|
|AOS1; HSPC140; SUA1; UBLE1A|
|The dimeric enzyme AOS1 acts as a an E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.|
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(LEFT)The AOS1 C-term Pab is used in Western blot to detect AOS1 in mouse heart tissue lysate. (RIGHT)Western blot analysis of AOS1 (arrow) using rabbit polyclonal AOS1 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the AOS1 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.