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Home Antibody All anti-RUVBL2 antibodies

Anti-RUVBL2 TRUEMAB Antibody Clone OTI1H10

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA504284 RUVBL2 (Reptin/TIP49B) mouse monoclonal antibody, clone OTI1H10 (formerly 1H10) 100ul $325 In Stock
LC401993 RUVBL2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF504284 Carrier-free (BSA/glycerol-free) RUVBL2 mouse monoclonal antibody, clone OTI1H10 (formerly 1H10) 100ug $450 3-4 weeks
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WB(1)
IHC(9)
IF(1)
FC(2)
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Also for RUVBL2 (NM_006666)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenHuman recombinant protein fragment corresponding to amino acids 113-370 of human RUVBL2(NP_006657) prduced in E.coli.
Clone NameClone OTI1H10 IsotypeIgG1
Species ReactivityHuman Concentration0.64 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100,
Predicted MW Explanation 51 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens RuvB-like AAA ATPase 2 (RUVBL2)
Alternative NameCGI-46; ECP51; INO80J; REPTIN; RVB2; TIH2; TIP48; TIP49B
Database LinkNP_006657
Entrez Gene 10856 Human
FunctionThis gene encodes the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this gene product has both ATPase and DNA helicase activities. This gene is physically linked to the CGB/LHB gene cluster on chromosome 19q13.3, and is very close (55 nt) to the LHB gene, in the opposite orientation. [provided by RefSeq].
Related PathwayTranscription Factors

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RUVBL2 (RC200933, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RUVBL2.
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-RUVBL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504284)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RUVBL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504284)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-RUVBL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504284)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RUVBL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504284)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-RUVBL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504284)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RUVBL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504284)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-RUVBL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504284)
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-RUVBL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504284)
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-RUVBL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504284)
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Anti-RUVBL2 mouse monoclonal antibody (TA504284) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RUVBL2(RC200933).
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HEK293T cells transfected with either RC200933 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RUVBL2 antibody(TA504284), and then analyzed by flow cytometry.
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Flow cytometric Analysis of Jurkat cells, using anti-RUVBL2 antibody(TA504284),(Red), compared to a nonspecific negative control antibody,(Blue).
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