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Anti-RUNX1 PHOSPHO Antibody


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SKU Description Amount Price Availability*  
TA313289 Rabbit polyclonal AML1 / RUNX1 (Ser435) antibody(Phospho-specific) 100ul 325
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OriGene Data

ImmunogenThe antiserum was produced against synthesized phosphopeptide derived from human AML1 around the phosphorylation site of serine 435 (S-N-SP-P-T).
Clone Name IsotypeIgG
Species ReactivityHuman (Identities = 100%, Positives = 100%); Mouse (Identities = 100%, Positives = 100%);Rat (Identities = 100%, Positives = 100%) Concentration1mg/ml
Guaranteed Application *WB Suggested DilutionsWB: 1:500~1:3000, ELISA: 1:10000
BufferPhosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Note AML1 (Phospho-Ser435) antibody detects endogenous levels of AML1 only when phosphorylated at serine 435.

Reference Data

Target NameHomo sapiens runt-related transcription factor 1 (RUNX1), transcript variant 1
Alternative NameAML1; AML1-EVI-1; AMLCR1; CBFA2; EVI-1; PEBP2aB
Database LinkNP_001745
Entrez Gene 861 Human
Entrez Gene 12394 Mouse
Entrez Gene 50662 Rat
Related PathwayES Cell Differentiation/IPSTranscription FactorsDruggable Genome Pathways in cancerChronic myeloid leukemiaAcute myeloid leukemia

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot analysis of extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins), using AML1 (Phospho-Ser435) antibody.The lane on the right is treated with the synthesized peptide.

WB Image
Western blot analysis of extracts from 293 cells (Lane 2), HeLa cells (Lane 3) and HepG2 cells (Lane 4), using AML1 (Phospho-Ser435) Antibody. The lane on the left is treated with synthesized peptide.


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