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Anti-RPS6KA5 Antibody EP1888Y
Also for RPS6KA5 (NM_182398)
|A synthetic phospho-peptide corresponding to residues surrounding Serine 360 of human MSK1 was used as immunogen. The antibody will detect MSK1 phosphorylation on Serine 360.|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
||ICC: 1:100 - 1:250; IP: 1:30; WB: 1:5000 - 1:20000
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for Flow Cyt or IHC-P.
|Homo sapiens ribosomal protein S6 kinase, 90kDa, polypeptide 5 (RPS6KA5), transcript variant 2|
|MSK1; MSPK1; RLPK|
|MSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress (1-2). Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term) (3). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain (4). |
EGFR1 Signaling Pathway
MAPK signaling pathway
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Western blot - MSK1 (phospho S360) antibody [EP1888Y]; All lanes : Anti-MSK1 (phospho S360) antibody [EP1888Y] at 1/20000 dilution.Lane 1 : HEK293 cell lyasate, untreated,.Lane 2 : HEK293 cell lyasate treated with AP.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti rabbit. at 1/2000 dilution.Predicted band size : 90 kDa.Observed band size : 90 kDa.
Immunocytochemistry/ Immunofluorescence - Anti-MSK1 (phospho S360) antibody [EP1888Y]; ICC/IF image of TA301030 stained MCF7 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4Â°C. The secondary antibody (green) was Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43ÂµM.