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Home Antibody All anti-RPS6KA5 antibodies

Anti-RPS6KA5 Antibody EP1888Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301030
  • Rabbit Monoclonal Antibody against RPS6KA5 (Clone EP1888Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC403633) , 20ug
100ul 325 3-7 Days
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WB(1)
IF(1)
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Also for RPS6KA5 (NM_182398)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic phospho-peptide corresponding to residues surrounding Serine 360 of human MSK1 was used as immunogen. The antibody will detect MSK1 phosphorylation on Serine 360.
Clone NameEP1888Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IF Suggested DilutionsICC: 1:100 - 1:250; IP: 1:30; WB: 1:5000 - 1:20000
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt or IHC-P.

Reference Data

Target NameHomo sapiens ribosomal protein S6 kinase, 90kDa, polypeptide 5 (RPS6KA5), transcript variant 2
Alternative NameMSK1; MSPK1; RLPK
Database LinkNP_872198
FunctionMSK-1 is a mitogen and stress activated protein kinase-1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK-1can be activated by ERK1/2 and SAPK2 /p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG-14 in response to mitogen and stress (1-2). Similar to RSK, MSK-1 contains two kinase domains (N-term and a C-term) (3). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK-1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C-terminal kinase domain of MSK-1 which is essential for the catalytic activity of the N-terminal kinase domain (4).
Related Pathway
EGFR1 Signaling Pathway
MAPK signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - MSK1 (phospho S360) antibody [EP1888Y]; All lanes : Anti-MSK1 (phospho S360) antibody [EP1888Y] at 1/20000 dilution.Lane 1 : HEK293 cell lyasate, untreated,.Lane 2 : HEK293 cell lyasate treated with AP.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti rabbit. at 1/2000 dilution.Predicted band size : 90 kDa.Observed band size : 90 kDa.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-MSK1 (phospho S360) antibody [EP1888Y]; ICC/IF image of TA301030 stained MCF7 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was DyLight488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.

 

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