RPE65 Mouse Monoclonal Antibody [Clone ID: 401.8B11.3D9]

CAT#: TA309839

Mouse Monoclonal RPE65 Antibody (401.8B11.3D9)

Product Images

Western Blot: RPE65 Antibody (401.8B11.3D9) - BSA Free TA309839 - WB analysis of RPE65 in 20ug lysate of COS7 cells expressing recombinant Human RPE65 (Lane 1) and 5ug of Bovine retinal pigment epithelium membrane fraction (Lane 2). Blot processed for detection with alkaline phosphatase conjugated goat-anti Mouse IgG secondary antibody and NBT/BCIP substrate.

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  Immunocytochemistry/Immunofluorescence: RPE65 Antibody (401.8B11.3D9) - BSA Free TA309839 - ICC-IF analysis of cultured ARPE19 cells, a spontaneously arising human retinal pigment epithelia cell line - 10 minutes fixation in 4% PFA, 10 minutes permeabilization in PBS containing 0.2% Triton X-100 (PBS-T), 1 hour blocking in 10% normal goat serum containing 1% BSA in PBS-T, 1:100 primary antibody dilution in PBS, ON 4C incubation.

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  Immunohistochemistry-Frozen: RPE65 Antibody (401.8B11.3D9) - BSA Free TA309839 - Staining of RPE65 in a cryosection of mouse retina tissue using RPE65 antibody (clone 401.8B11.3D9).

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  Immunohistochemistry-Paraffin: RPE65 Antibody (401.8B11.3D9) - BSA Free TA309839 - Analysis of FFPE human glioblastoma tissue section using 1:500 dilution of RPE65 [401.8B11.3D9] antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems).

Specifications

Product Data

• Clone Name: 401.8B11.3D9
• Applications: CyTOF-ready, FC, ICC/IF, IHC, IP, Simple Western, WB
• Recommended Dilution: Immunohistochemistry-Paraffin: 1:250-1:500, Immunohistochemistry-Frozen: 1:250, Simple Western, Immunohistochemistry: 1:250, Knockout Validated, Immunoprecipitation, Flow Cytometry, Western Blot: 1 - 2 ug/ml, Immunocytochemistry/ Immunofluorescence: 1:50 - 1:200, Immunofluorescence: 1:50 - 1:200, CyTOF-ready
• Reactivities: Bovine, Human, Mouse, Porcine
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: Bovine RPE microsomal membrane proteins.
• Formulation: PBS and 0.1% Sodium Azide
• Concentration: lot specific
• Purification: Protein A purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: retinal pigment epithelium-specific protein 65kDa
• Database Link:
  NP_000320
  Entrez Gene 19892 MouseEntrez Gene 6121 Human
  UniProt ID Q16518
• Synonyms: BCO3; LCA2; mRPE65; rd12; RP20; sRPE65

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Retinol metabolism