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OriGene Antibodies in recent publications
A common polymorphism in the LDL receptor gene has multiple effects on LDL receptor function Hum. Mol. Genet., Apr 2013; 22: 1424 - 1431. [anti-DDK]
Excitotoxicity Upregulates SARM1 Protein Expression and Promotes Wallerian-Like Degeneration of Retinal Ganglion Cells and Their Axons Invest. Ophthalmol. Vis. Sci., Apr 2013; 54: 2771 - 2780. [SARM1]
Modulation of TET2 expression and 5-methylcytosine oxidation by the CXXC domain protein IDAX Nature 497, 122-126 doi:10.1038/nature12052 [anti-DDK]
Natriuretic Peptide Receptor-3 Gene (NPR3): Nonsynonymous Polymorphism Results in Significant Reduction in Protein Expression Because of Accelerated Degradation Circ Cardiovasc Genet, Apr 2013; 6: 201 - 210. [NPR3]
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 Home All anti-RPA2 antibodies

Anti-RPA2 TrueMAB Antibody clone 8B3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Related Products Conjugation/Bulk FAQs
SKU Description Amount Price Availability*  
TA500788
  • Purified RPA2 mouse monoclonal antibody, clone 8B3
  • FREE positive control: HEK293T cell transient overexpression lysate (LC401031) , 20ug
 100ul $325 In Stock Add to Shopping Cart
CF500788
  • Carrier-free (BSA/glycerol-free) RPA2 mouse monoclonal antibody, clone 8B3
100ug $450 3-4 weeks Add to Shopping Cart
WB(1)
IP(1)
IHC(9)
IF(2)
FC(2)
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Also for RPA2 (NM_002946)
cDNA Clone shRNA/siRNA Lysate Protein Antibody
Gene NameHomo sapiens replication protein A2, 32kDa (RPA2)
Synonyms:REPA2; RPA32
ImmunogenFull length human recombinant protein of human RPA2 (NP_002937) produced in HEK293T cell.
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clone Nameclone 8B3 IsotypeIgG1
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Purification Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
Guaranteed Application *WB, IHC, IF, IP, FC Suggested DilutionsWB 1:4000, IHC 1:50, IF 1:50~100, FLOW 1:100, IP 2ug/500ul
Background
Related Pathway
Cell cycle

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RPA2 (RC205715, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RPA2.
IHC Image
Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-RPA2 mouse monoclonal antibody. (TA500788)
IHC Image
Immunohistochemical staining of paraffin-embedded Human colon tissue using anti-RPA2 mouse monoclonal antibody. (TA500788)
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-RPA2 mouse monoclonal antibody. (TA500788)
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-RPA2 mouse monoclonal antibody. (TA500788)
IHC Image
Immunohistochemical staining of paraffin-embedded Human Ovary tissue using anti-RPA2 mouse monoclonal antibody. (TA500788)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RPA2 mouse monoclonal antibody. (TA500788)
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-RPA2 mouse monoclonal antibody. (TA500788)
IHC Image
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-RPA2 mouse monoclonal antibody. (TA500788)
IHC Image
Immunohistochemical staining of paraffin-embedded Human bladder tissue using anti-RPA2 mouse monoclonal antibody. (TA500788)
IF Image
Anti-RPA2 mouse monoclonal antibody (TA500788) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RPA2(RC205715).
IF Image
Immunofluorescent staining of HepG2 cells using anti-RPA2 mouse monoclonal antibody (TA500788).
IP Image
Immunoprecipitation(IP) of RPA2 by using TrueMab monoclonal anti-RPA2 antibodies (Negative control: IP without adding anti-RPA2 antibody.). For each experiment, 500ul of DDK tagged RPA2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RPA2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
FC Image
Flow cytometric Analysis of Hela cells, using anti-RPA2 antibody(TA500788),(Red), compared to a nonspecific negative control antibody,(Blue).
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-RPA2 antibody(TA500788),(Red), compared to a nonspecific negative control antibody,(Blue).

 

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