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Home Antibody All anti-RPA2 antibodies

Anti-RPA2 Antibody EPR2877Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA301295
  • Rabbit Monoclonal Antibody against RPA2 (Clone EPR2877Y)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC401031) , 20ug
100ul $325 3-7 Days
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WB(1)
IHC(1)
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Also for RPA2 (NM_002946)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues on the C terminus of human RPA32 was used as an immunogen.
Clone NameEPR2877Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC Suggested DilutionsWB: 1:5,000~10,000; IHC: 1:250~500; IP: 1:40
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens replication protein A2, 32kDa (RPA2), transcript variant 1
Alternative NameREPA2; RPA32
Database LinkNP_002937
FunctionReplication protein A (RPA) is a heterotrimeric single-stranded DNA-binding protein that plays essential roles in nucleic acid metabolism, including DNA replication, nucleotide excision repair, and homologous recombination. It is a complex of three polypeptides of molecular mass 70, 32, and 14 kDa (RPA70, RPA32, and RPA14). RPA is a phosphorylation target for DNA-dependent protein kinase (DNA-PK) and likely the ataxia telangiectasia-mutated gene (ATM) protein kinase. The middle subunit, RPA32, is phosphorylated in a cell cycle-dependent manner which begins in parallel to the degradation of DNA to high molecular weight fragments, and slowly continues until late apoptosis. Hyperphosphorylation of RPA32 requires the activities of DNA-dependent protein kinase and of a cyclin-dependent protein kinase (1-3). RPA32 has been reported to be a useful prognostic indicator in colon cancer patients and an attractive therapeutic target for regulation by tumor suppressors or other proteins involved in the control of cell proliferation (4).
Related Pathway
Cell cycle

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WB Image
Western blot - RPA32/RPA2 antibody [EPR2877Y]; All lanes : Anti-RPA32/RPA2 antibody [EPR2877Y] at 1/10000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : HUVEC lysate.Lane 3 : NIH 3T3 cell lysate.Lane 4 : C6 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/1000 dilution.Predicted band size : 29 kDa.Observed band size : 29 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RPA32/RPA2 antibody [EPR2877Y]; Immunohistochemical analysis of paraffin-embedded human breast carcinoma using TA301295 at 1/250 dilution.

 

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