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Anti-RPA2 Antibody EPR2877Y
Also for RPA2 (NM_002946)
|A synthetic peptide corresponding to residues on the C terminus of human RPA32 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:5,000~10,000; IHC: 1:250~500; IP: 1:40
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Homo sapiens replication protein A2, 32kDa (RPA2), transcript variant 1|
|Replication protein A (RPA) is a heterotrimeric single-stranded DNA-binding protein that plays essential roles in nucleic acid metabolism, including DNA replication, nucleotide excision repair, and homologous recombination. It is a complex of three polypeptides of molecular mass 70, 32, and 14 kDa (RPA70, RPA32, and RPA14). RPA is a phosphorylation target for DNA-dependent protein kinase (DNA-PK) and likely the ataxia telangiectasia-mutated gene (ATM) protein kinase. The middle subunit, RPA32, is phosphorylated in a cell cycle-dependent manner which begins in parallel to the degradation of DNA to high molecular weight fragments, and slowly continues until late apoptosis. Hyperphosphorylation of RPA32 requires the activities of DNA-dependent protein kinase and of a cyclin-dependent protein kinase (1-3). RPA32 has been reported to be a useful prognostic indicator in colon cancer patients and an attractive therapeutic target for regulation by tumor suppressors or other proteins involved in the control of cell proliferation (4). |
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Western blot - RPA32/RPA2 antibody [EPR2877Y]; All lanes : Anti-RPA32/RPA2 antibody [EPR2877Y] at 1/10000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : HUVEC lysate.Lane 3 : NIH 3T3 cell lysate.Lane 4 : C6 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/1000 dilution.Predicted band size : 29 kDa.Observed band size : 29 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RPA32/RPA2 antibody [EPR2877Y]; Immunohistochemical analysis of paraffin-embedded human breast carcinoma using TA301295 at 1/250 dilution.