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Anti-RPA1 Antibody EPR3473
Also for RPA1 (NM_002945)
|A synthetic peptide corresponding to residues in human RPA 70 was used as an immunogen.|
||Tissue culture supernatant
||Lot dependent; please refer to CoA along with shipment
||WB: 1:10000 - 1:50000; IP: 1:50; FC: 1:100
|Does not react with Mouse, Rat. Is unsuitable for ICC/IF or IHC-P.|
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Is unsuitable for ICC/IF or IHC-P.
|Homo sapiens replication protein A1, 70kDa (RPA1)|
|HSSB; MST075; REPA1; RF-A; RP-A; RPA70|
|Replication protein A (RPA) is a single-stranded DNA-binding (ssDNA) protein involved in multiple processes of eukaryotic DNA metabolism, including DNA replication, repair, and recombination. It is found to be essential for replication of the papovavirus SV40 (1). RPA is a complex of three polypeptides of 70, 34, and 13 kDa (2). The DNA-binding activity may reside exclusively on the 70-kDa subunit RPA 70. The 70-kDa subunit of RPA (RPA 70) is composed of three functional domains consisting of an N-terminal domain that is not required for ssDNA binding or SV40 replication, a central DNA-binding domain, and a C-terminal domain that is essential for subunit interactions (3). |
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Western blot - Anti-RPA70 antibody [EPR3473]; All lanes : Anti-RPA70 antibody [EPR3473] at 1/10000 dilution.Lane 1 : A431 cell lysate.Lane 2 : HeLa cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 68 kDa.Observed band size : 70 kDa .