OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-RPA1 antibodies

Anti-RPA1 Antibody EPR3472

div

Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA307403
  • Rabbit monoclonal antibody against RPA 70 (clone EPR3472 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401030) , 20ug Explanation
100ul 325 3-7 Days
Add to Shopping Cart
spacer
WB(1)
IHC(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human RPA 70 was used as an immunogen.
Clone NameEPR3472 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:2000 - 1:5000; IP: 1:10; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:50; RIP: Use at an assay dependent concentration
Predicted MW Explanation 70 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens replication protein A1, 70kDa (RPA1)
Alternative NameHSSB; MST075; REPA1; RF-A; RP-A; RPA70
Database LinkNP_002936
Entrez Gene 6117 Human
FunctionReplication protein A (RPA) is a single-stranded-DNA-binding protein involved in multiple processes of eukaryotic DNA metabolism, including DNA replication, repair, and recombination, and found to be essential for replication of the papovavirus SV40 (1). It is a complex of three polypeptides of 70, 34, and 13 kDa (2). The 70-kDa subunit of RPA (RPA 70) is composed of three functional domains consisting of an N-terminal domain that is not required for ssDNA binding or SV40 replication, a central DNA-binding domain, and a C-terminal domain that is essential for subunit interactions (3).
Related PathwayStem cell - PluripotencyDruggable Genome DNA replicationNucleotide excision repairMismatch repairHomologous recombination

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - RPA70 antibody [EPR3472]; All lanes : Anti-RPA70 antibody [EPR3472] at 1/5000 dilution.Lane 1 : A549 cell lysate.Lane 2 : HeLa cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 70 kDa.Observed band size : 70 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RPA70 antibody [EPR3472]; TA307403 at 1/100 dilution staining RPA70 in human cervical squamous cell carcinoma by Immunohistochemistry using paraffin-embedded tissue.
FC Image
Flow Cytometry-Anti-RPA70 antibody [EPR3472](TA307403); Overlay histogram showing HeLa cells stained with TA307403 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

spacer
Inc 5000 Healthcare Company Copyright © 2015 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title