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Anti-RPA1 Antibody EPR3472
Also for RPA1 (NM_002945)
|A synthetic peptide corresponding to residues in human RPA 70 was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, FC
||WB: 1:2000 - 1:5000; IP: 1:10; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:50; RIP: Use at an assay dependent concentration
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens replication protein A1, 70kDa (RPA1)|
|HSSB; MST075; REPA1; RF-A; RP-A; RPA70|
|Replication protein A (RPA) is a single-stranded-DNA-binding protein involved in multiple processes of eukaryotic DNA metabolism, including DNA replication, repair, and recombination, and found to be essential for replication of the papovavirus SV40 (1). It is a complex of three polypeptides of 70, 34, and 13 kDa (2). The 70-kDa subunit of RPA (RPA 70) is composed of three functional domains consisting of an N-terminal domain that is not required for ssDNA binding or SV40 replication, a central DNA-binding domain, and a C-terminal domain that is essential for subunit interactions (3). |
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Western blot - RPA70 antibody [EPR3472]; All lanes : Anti-RPA70 antibody [EPR3472] at 1/5000 dilution.Lane 1 : A549 cell lysate.Lane 2 : HeLa cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 70 kDa.Observed band size : 70 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RPA70 antibody [EPR3472]; ab79398 at 1/100 dilution staining RPA70 in human cervical squamous cell carcinoma by Immunohistochemistry using paraffin-embedded tissue.
Flow Cytometry-Anti-RPA70 antibody [EPR3472](ab79398); Overlay histogram showing HeLa cells stained with ab79398 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.