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Home Antibody All anti-RPA1 antibodies

Anti-RPA1 Antibody EPR3472

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307403
  • Rabbit monoclonal antibody against RPA 70 (clone EPR3472 )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401030) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(1)
FC(1)
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Also for RPA1 (NM_002945)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human RPA 70 was used as an immunogen.
Clone NameEPR3472 IsotypeIgG
Species ReactivityHuman (Does not react with: Mouse, Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:2000 - 1:5000; IP: 1:10; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250; FC: 1:50; RIP: Use at an assay dependent concentration
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens replication protein A1, 70kDa (RPA1)
Alternative NameHSSB; MST075; REPA1; RF-A; RP-A; RPA70
Database LinkNP_002936
Entrez Gene 6117 Human
FunctionReplication protein A (RPA) is a single-stranded-DNA-binding protein involved in multiple processes of eukaryotic DNA metabolism, including DNA replication, repair, and recombination, and found to be essential for replication of the papovavirus SV40 (1). It is a complex of three polypeptides of 70, 34, and 13 kDa (2). The 70-kDa subunit of RPA (RPA 70) is composed of three functional domains consisting of an N-terminal domain that is not required for ssDNA binding or SV40 replication, a central DNA-binding domain, and a C-terminal domain that is essential for subunit interactions (3).
Related Pathway
Cell cycle

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - RPA70 antibody [EPR3472]; All lanes : Anti-RPA70 antibody [EPR3472] at 1/5000 dilution.Lane 1 : A549 cell lysate.Lane 2 : HeLa cell lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 70 kDa.Observed band size : 70 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RPA70 antibody [EPR3472]; TA307403 at 1/100 dilution staining RPA70 in human cervical squamous cell carcinoma by Immunohistochemistry using paraffin-embedded tissue.
FC Image
Flow Cytometry-Anti-RPA70 antibody [EPR3472](TA307403); Overlay histogram showing HeLa cells stained with TA307403 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed.

 

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