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Home Antibody All anti-ROCK1 antibodies

Anti-ROCK1 Antibody EPR638Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303689
  • Rabbit monoclonal antibody against ROCK1 (EPR638Y )
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC417325) , 20ug Explanation
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human ROCK1 was used as an immunogen.
Clone NameEPR638Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsICC/IF: Use at an assay dependent concentration; WB: 1:500 - 1:1000; IP: 1:60; ICC: 1:50 - 1:100; IHC-P: 1:50 - 1:100
Predicted MW Explanation 158 kDa
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens Rho-associated, coiled-coil containing protein kinase 1 (ROCK1)
Alternative NameP160ROCK; ROCK-I
Database LinkNP_005397
Entrez Gene 6093 Human
Entrez Gene 19877 Mouse
Entrez Gene 81762 Rat
FunctionThis gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq].
Related PathwayProtein KinaseDruggable Genome Chemokine signaling pathwayVascular smooth muscle contractionWnt signaling pathwayTGF-beta signaling pathwayAxon guidanceFocal adhesionMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Anti-ROCK1 antibody [EPR638Y]; Anti-ROCK1 antibody [EPR638Y] at 1/500 dilution + HeLa cell lysate at 10 ug.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 158 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK1 antibody [EPR638Y]; Immunohistochemical analysis of paraffin embedded Human lymphoma tissue labelling ROCK1 with TA303689 at a dilution of 1/50.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-ROCK1 antibody [EPR638Y]; TA303689 (1/200) staining ROCK1 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red).

 

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