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Anti-ROCK1 Antibody EPR638Y
Also for ROCK1 (NM_005406)
|A synthetic peptide corresponding to residues near the C-terminus of human ROCK1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||ICC/IF: Use at an assay dependent concentration; WB: 1:500 - 1:1000; IP: 1:60; ICC: 1:50 - 1:100; IHC-P: 1:50 - 1:100
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens Rho-associated, coiled-coil containing protein kinase 1 (ROCK1)|
|This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. [provided by RefSeq]. |
Signaling by GPCR
TGF Beta Signaling Pathway
Wnt Signaling Pathway
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Western blot - Anti-ROCK1 antibody [EPR638Y]; Anti-ROCK1 antibody [EPR638Y] at 1/500 dilution + HeLa cell lysate at 10 µg.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 158 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK1 antibody [EPR638Y]; Immunohistochemical analysis of paraffin embedded Human lymphoma tissue labelling ROCK1 with ab134181 at a dilution of 1/50.
Immunocytochemistry/ Immunofluorescence - Anti-ROCK1 antibody [EPR638Y]; ab134181 (1/200) staining ROCK1 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.