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Anti-RNF144B TRUEMAB Antibody Clone OTI6B11
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for RNF144B (NM_182757)
|Full length human recombinant protein of human RNF144B (NP_877434) produced in HEK293T cell.|
|WB, IF, FC
||WB 1:500, IF 1:100, FLOW 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RNF144B (RC209302, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RNF144B.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RNF144B monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
Anti-RNF144B mouse monoclonal antibody (TA500722) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RNF144B(RC209302 ).
HEK293T cells transfected with either RC209302 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-RNF144B antibody(TA500722), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-RNF144B antibody(TA500722),(Red), compared to a nonspecific negative control antibody,(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-RNF144B antibody(TA500722),(Red), compared to a nonspecific negative control antibody,(Blue).