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Home Antibody All anti-RHOJ antibodies

Anti-RHOJ TRUEMAB Antibody Clone OTI2C10

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA505466 RHOJ mouse monoclonal antibody,clone OTI2C10 100ul
30ul
$325 In Stock
LC412401 RHOJ HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF505466 Carrier-free (BSA/glycerol-free) RHOJ mouse monoclonal antibody,clone OTI2C10 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(2)
IHC(3)
IF(1)

OriGene Data

ImmunogenFull length human recombinant protein of human RHOJ(NP_065714) produced in HEK293T cell.
Clone NameClone OTI2C10 IsotypeIgG2a
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF Suggested Dilutions WB 1:2000, IHC 1:150, IF 1:100,
Predicted MW Explanation 23.6 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens ras homolog family member J (RHOJ)
Alternative NameARHJ; RASL7B; TC10B; TCL
Database LinkNP_065714
Entrez Gene 57381 Human
FunctionThis gene encodes one of the many small GTP-binding proteins in the Rho family shown to be associated with focal adhesions in endothelial cells (PMID: 21148427, 22103495). The encoded protein is activated by vascular endothelial growth factor and may regulate angiogenesis. [provided by RefSeq, Dec 2011].
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RHOJ (RC209395, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RHOJ.
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Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-RHOJ monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-RHOJ mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505466)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RHOJ mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505466)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-RHOJ mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505466)
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Anti-RHOJ mouse monoclonal antibody (TA505466) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RHOJ(RC209395).
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