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Anti-RGS9 Antibody EPR2873
Also for RGS9 (NM_001081955)
|A synthetic peptide corresponding to residues in human RGS9 was used as an immunogen|
|Human, Mouse, Rat
||Lot dependent; please refer to CoA along with shipment
||WB: 1:1000; IP: 1:10; ICC: 1:50 - 1:100; FC: 1:30
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for IHC-P.
|Homo sapiens regulator of G-protein signaling 9 (RGS9), transcript variant 2|
|Regulator of G-protein signaling 9 (RGS9) is a GTPase-activating protein. It enhances the time resolution of G protein signaling cascades by accelerating GTP hydrolysis of G alpha subunits of heterotrimeric G proteins (1, 2). RGS9 deactivates the G proteins (transducins) in the rod and cone phototransduction cascades. It is anchored to photoreceptor membranes by the transmembrane protein R9AP (RGS9 anchor protein), which enhances RGS9 activity up to 70-fold (3). As a peripheral membrane protein, RGS9 is highly expressed in the caudate and putamen and lower levels are found in the hypothalamus, nucleus accumbens and cerebellum. Defects in RGS9 are suggested as the cause of prolonged electroretinal response suppression, also known as bradyopsia (4).|
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Western blot - RGS9 antibody [EPR2873]; All lanes : Anti-RGS9 antibody [EPR2873] at 1/1000 dilution.Lane 1 : PC12 lysates.Lane 2 : Human spleen lysates.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 77 kDa.Observed band size : 55 kDa .
Flow Cytometry - RGS9 antibody [EPR2873]; Flow cytometric analysis of RGS9 in permeabilized PC12 cells, using TA307611 (red) at a 1/30 dilution or a rabbit IgG (negative) (green).