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Home Antibody All anti-RFXANK antibodies

Anti-RFXANK TRUEMAB Antibody Clone 3F5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA504270
  • RFXANK mouse monoclonal antibody,clone 3F5
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC408725) , 20ug Explanation
100ul 325 In Stock
CF504270
  • Carrier-free (BSA/glycerol-free) RFXANK mouse monoclonal antibody,clone 3F5
100ug $450 3-4 weeks
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(1)
IHC(4)
IF(1)
FC(1)
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Also for RFXANK (NM_134440)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human RFXANK(NP_604389) produced in HEK293T cell.
Clone NameClone 3F5 IsotypeIgG1
Species ReactivityHuman Concentration0.76 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100,
Predicted MW Explanation 25.4 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens regulatory factor X-associated ankyrin-containing protein (RFXANK), transcript variant 2
Alternative NameANKRA1; BLS; F14150_1; RFX-B
Database LinkNP_604389
Entrez Gene 8625 Human
FunctionMajor histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. This protein contains ankyrin repeats involved in protein-protein interactions. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group B. Two transcript variants encoding different isoforms have been described for this gene, with only one isoform showing activation activity. [provided by RefSeq].
Related PathwayTranscription FactorsDruggable Genome Antigen processing and presentationPrimary immunodeficiency

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFXANK (RC223081, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFXANK.
IHC Image
Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-RFXANK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504270)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RFXANK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504270)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RFXANK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504270)
IHC Image
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-RFXANK mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504270)
IF Image
Anti-RFXANK mouse monoclonal antibody (TA504270) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RFXANK(RC223081).
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-RFXANK antibody(TA504270),(Red), compared to a nonspecific negative control antibody,(Blue).

 

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