Anti-RFC4 Antibody, Clone 1A8

Also for RFC4 (NM_002916)

OriGene Data

Immunogen	Full length human recombinant protein of human RFC4(NP_002907) produced in HEK293T cell.
Clone Name	Clone 1A8	Isotype	IgG1
Species Reactivity	Human , Dog , Rat , Monkey , Mouse	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, IF	Suggested Dilutions	WB 1:200~4000, IHC 1:150, IF 1:100,
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens replication factor C (activator 1) 4, 37kDa (RFC4), transcript variant 1
Alternative Name	A1; RFC37
Database Link	NP_002907
Function	The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008].
Related Pathway	Cell cycle

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC4 (RC200426, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFC4.

WB Image

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RFC4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

IHC Image

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-RFC4 mouse monoclonal antibody. (TA506183)

IHC Image

Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-RFC4 mouse monoclonal antibody. (TA506183)

IHC Image

Immunohistochemical staining of paraffin-embedded Human tonsil using anti-RFC4 mouse monoclonal antibody. (TA506183)

IF Image

Anti-RFC4 mouse monoclonal antibody (TA506183) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RFC4(RC200426).

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Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

Anti-RFC4 TrueMAB Antibody Clone 1A8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data