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Home Antibody All anti-RFC4 antibodies

Anti-RFC4 TRUEMAB Antibody Clone 1A8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA506183
  • RFC4 mouse monoclonal antibody,clone 1A8
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC419019) , 20ug Explanation
100ul 379 In Stock
CF506183
  • Carrier-free (BSA/glycerol-free) RFC4 mouse monoclonal antibody,clone 1A8
100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
This antibody qualifies for Season of Giving promotion: Earn $50 Amazon gift card (use promo code SPQ12081) or donation to Cancer.org (promo code SPQ12082) with $600 purchase of UltraMAB or TrueMAB. Click for details
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WB(2)
IHC(3)
IF(1)
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Also for RFC4 (NM_002916)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human RFC4(NP_002907) produced in HEK293T cell.
Clone NameClone 1A8 IsotypeIgG1
Species ReactivityHuman , Dog , Rat , Monkey , Mouse Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF Suggested Dilutions WB 1:200~4000, IHC 1:150, IF 1:100,
Predicted MW Explanation 39.5 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens replication factor C (activator 1) 4, 37kDa (RFC4), transcript variant 1
Alternative NameA1; RFC37
Database LinkNP_002907
Entrez Gene 5984 Human
Entrez Gene 106344 Mouse
Entrez Gene 288003 Rat
Entrez Gene 100426721 Monkey
Entrez Gene 478667 Dog
FunctionThe elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008].
Related PathwayStem cell - PluripotencyDruggable Genome DNA replicationNucleotide excision repairMismatch repair

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RFC4 (RC200426, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RFC4.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RFC4 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-RFC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506183)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-RFC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506183)
IHC Image
Immunohistochemical staining of paraffin-embedded Human tonsil using anti-RFC4 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA506183)
IF Image
Anti-RFC4 mouse monoclonal antibody (TA506183) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RFC4(RC200426).

 

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