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Home Antibody All anti-RAPGEF1 antibodies

Anti-RAPGEF1 TRUEMAB Antibody Clone 2F5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA506837
  • RAPGEF1 mouse monoclonal antibody,clone 2F5
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC404802) , 20ug
100ul 325 In Stock
CF506837
  • Carrier-free (BSA/glycerol-free) RAPGEF1 mouse monoclonal antibody,clone 2F5
100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(2)
IHC(7)
IF(1)
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Also for RAPGEF1 (NM_198679)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human RAPGEF1(NP_941372) produced in HEK293T cell.
Clone NameClone 2F5 IsotypeIgG1
Species ReactivityHuman , Dog , Rat , Monkey , Mouse Concentration1mg/ml
Guaranteed Application *WB, IHC, IF Suggested Dilutions WB 1:400~4000, IHC 1:150, IF 1:100,
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens Rap guanine nucleotide exchange factor (GEF) 1 (RAPGEF1), transcript variant 2
Alternative NameC3G; GRF2
Database LinkNP_941372
FunctionThis gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008].
Related Pathway
Focal Adhesion

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RAPGEF1 (RC208092, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RAPGEF1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RAPGEF1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
IHC Image
Immunohistochemical staining of paraffin-embedded Human breast tissue using anti-RAPGEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA506837)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-RAPGEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA506837)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-RAPGEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA506837)
IHC Image
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-RAPGEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA506837)
IHC Image
Immunohistochemical staining of paraffin-embedded Human thyroid tissue using anti-RAPGEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA506837)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-RAPGEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA506837)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-RAPGEF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA506837)
IF Image
Anti-RAPGEF1 mouse monoclonal antibody (TA506837) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RAPGEF1(RC208092).

 

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