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Anti-RAB4A Antibody EPR3042
Also for RAB4A (NM_004578)
|A synthetic peptide corresponding to residues in human Rab4 was used as an immunogen.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1:2000 - 1:10000; FC: 1:150; ICC/IF: 1:100 - 1:250
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
|Is unsuitable for IHC-P or IP.
|Homo sapiens RAB4A, member RAS oncogene family (RAB4A), transcript variant 1|
|HRES-1; HRES-1/RAB4; HRES1; RAB4|
Entrez Gene 5867 Human
Entrez Gene 19341 Mouse
Entrez Gene 25532 Rat
|Rab proteins constitute a family of GTP-binding proteins that are located in distinct intracellular compartments and play a role in the regulation of vesicular trafficking (1). The small GTPase Rab4 is associated with early endocytic compartments and regulates receptor recycling from early endosomes. It has been proposed that Rab4 assists in the docking of transport vesicles en route from early endosomes to recycling endosomes (2). Rab4 (along with Rab5 and Rab11) regulate sequential transport steps along the endocytic/recycling pathway, and occupy distinct membrane domains on early and recycling endosomes. It seems that divalent Rab effectors regulate protein sorting and recycling by connecting Rab domains on early endosomes (3).|
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Western blot - Rab4 antibody [EPR3042]; All lanes : Anti-Rab4 antibody [EPR3042] at 1/2000 dilution.Lane 1 : MCF7 cell lysates .Lane 2 : PC12 cell lysates .Lane 3 : Fetal brain cell lysates .Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 24 kDa.Observed band size : 25 kDa .
Immunocytochemistry/ Immunofluorescence - Rab4 antibody [EPR3042]; Immunofluorescent staining of Rab4 in HeLa cells, using TA307279 at a 1/100 dilution.