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Home Antibody All anti-PYCARD antibodies

Anti-PYCARD TRUEMAB Antibody Clone OTI1A2

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA800079 PYCARD (TMS1) mouse monoclonal antibody, clone OTI1A2 (formerly 1A2) 100ul $325 In Stock
LC402233 PYCARD HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF800079 Carrier-free (BSA/glycerol-free) PYCARD mouse monoclonal antibody, clone OTI1A2 (formerly 1A2) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type

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WB(2)
FC(1)
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Also for PYCARD (NM_013258)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human PYCARD (NP_037390) produced in E.coli.
Clone NameClone OTI1A2 IsotypeIgG1
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, FC Suggested DilutionsWB 1:500~2000, FLOW 1:100,
Predicted MW Explanation 21.4 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens PYD and CARD domain containing (PYCARD), transcript variant 1
Alternative NameASC; CARD5; TMS; TMS-1; TMS1
Database LinkNP_037390
Entrez Gene 29108 Human
FunctionThis gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Related PathwayDruggable Genome NOD-like receptor signaling pathwayCytosolic DNA-sensing pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCARD (RC215592, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCARD.
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Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PYCARD monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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HEK293T cells transfected with either RC215592 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PYCARD antibody(TA800079), and then analyzed by flow cytometry.
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