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Home All anti-PYCARD antibodies
Anti-PYCARD TrueMAB Antibody Clone 1A2
TrueMAB™ Antibodies - Made against Authentic Protein Antigens
* Buy one primary antibody, get 50% off a GAPDH loading control antibody.
Also for PYCARD (NM_013258)
OriGene Data
| Full length human recombinant protein of human PYCARD (NP_037390) produced in E.coli. |
| Clone 1A2 |
IgG1 |
| Human |
0.5~1.0 mg/ml (Lot Dependent) |
| WB, FC |
WB 1:500~2000, FLOW 1:100, |
| PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
| Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose) |
Reference Data
| Homo sapiens PYD and CARD domain containing (PYCARD), transcript variant 1 |
| ASC; CARD5; TMS; TMS-1; TMS1 |
| NP_037390 |
| This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. |
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* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.
WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCARD (RC215592, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCARD.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-PYCARD monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
FC Image
HEK293T cells transfected with either RC215592 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PYCARD antibody(TA800079), and then analyzed by flow cytometry.
Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:
- HRP
- Biotin
- PE
- FITC
- DyLight® 488
- DyLight® 550
- DyLight® 594
- DyLight® 633
* DyLight is a trademark of Thermo-Fisher Scientific
Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.
Contact us with your conjugation/bulk request: antibody@origene.com or 888-267-4436 (Tech Support)
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