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Anti-PXN PHOSPHO Antibody


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA323770 Anti-PXN (Phospho-Tyr31) Rabbit Polyclonal Antibody 100ul 325
2-3 Weeks
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OriGene Data

ImmunogenPeptide sequence around phosphorylation site of tyrosine 31 (T-P-Y(p)-S-Y) derived from Human Paxillin.
Clone Name IsotypeIgG
Species ReactivityHuman; Mouse; Rat Concentration1mg/ml
Guaranteed Application *WB Suggested DilutionsWB: 1:500-1000
Predicted MW Explanation 65kDa
BufferPBS pH7.3, 0.05% NaN3, 50% glycerol
Purification Antigen affinity purification

Reference Data

Target NameHomo sapiens paxillin (PXN), transcript variant 4
Alternative NameFLJ16691
Database LinkNP_079433
Entrez Gene 5829 Human
Entrez Gene 19303 Mouse
Entrez Gene 360820 Rat
FunctionAn antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions
Related PathwayStem cell - PluripotencyDruggable Genome Chemokine signaling pathwayVEGF signaling pathwayFocal adhesionLeukocyte transendothelial migrationRegulation of actin cytoskeleton

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Predicted band size: 65 kDa . Positive control: HT29 cells treated with PMA lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: HT29 cells untreated with PMA lysate Lane2: HT29 cells treated with PMA lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)


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